ERK1 recombinant protein

ERK1, Active

Cat. Num. AAA515543

• Gene Names: MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
• Applications: Western Blot
• Synonyms: ERK1; Active; ERK1 recombinant protein

• Host: Sf9 insect cells using baculovirus
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Sequence Positions: Full Length
• Sequence Length: 1902

• Applicable Applications for ERK1 recombinant protein: Kinase Assay, Western Blot (WB)
• Species: Human
• Tag: Tag-free
• Preparation and Storage: Store product at -70 degree C.; For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.; For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Testing Data

Testing Data #2

Related Product Information for ERK1 recombinant protein

Description: Recombinant full-length, tag-free human ERK1 was by baculovirus in Sf9 insect cells and activated by active MEK1in vitro.
Scientific Background: ERK1 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos, and C/EBP beta).

Product Categories/Family for ERK1 recombinant protein

Enzymes; Active Kinases

References

1. Boulton, TG. et al: Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase.
Biochemistry. 1991 Jan 8;30 (1):278-86.

NCBI and Uniprot Product Information

• NCBI GI #: 91718898
• NCBI GeneID: 5595
• NCBI Accession #: NM_002746.2
• NCBI GenBank Nucleotide #: NM_002746
• UniProt Accession #: P27361; Q8NHX1; A8CZ58; B0LPG3
• Molecular Weight: ~44 kDa
• NCBI Official Full Name: Homo sapiens mitogen-activated protein kinase 3 (MAPK3), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 3
• NCBI Official Symbol: MAPK3
• NCBI Official Synonym Symbols: ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
• NCBI Protein Information: mitogen-activated protein kinase 3
• UniProt Protein Name: Mitogen-activated protein kinase 3
• UniProt Gene Name: MAPK3
• UniProt Synonym Gene Names: ERK1; PRKM3; MAP kinase 3; MAPK 3; ERK-1; p44-MAPK

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

ERK1: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates.

Protein type: CMGC group; EC 2.7.11.24; ERK subfamily; Kinase, protein; MAPK family; MAPK/ERK subfamily; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: caveola; cytoplasm; cytoskeleton; cytosol; early endosome; focal adhesion; Golgi apparatus; late endosome; mitochondrion; nuclear envelope; nucleoplasm; nucleus; plasma membrane

Molecular Function: kinase activity; MAP kinase activity; phosphatase binding; protein binding; protein serine/threonine kinase activity

Biological Process: activation of MAPK activity; axon guidance; BMP signaling pathway; cellular response to amino acid starvation; DNA damage induced protein phosphorylation; fibroblast growth factor receptor signaling pathway; MAPKKK cascade; phosphorylation; platelet activation; positive regulation of cyclase activity; positive regulation of histone acetylation; positive regulation of histone phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of cytoskeleton organization and biogenesis; regulation of phosphoinositide 3-kinase cascade; regulation of stress-activated MAPK cascade; regulation of transcription factor activity; stress-activated MAPK cascade; transcription initiation from RNA polymerase I promoter