Cystatin C / CST3 Recombinant Protein | CST3 recombinant protein

Recombinant Rat Cystatin C / CST3 Protein (His tag)

Cat. Num. AAA2547487

• Gene Names: Cst3; CYSC
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: Cystatin C / CST3; Recombinant Rat Cystatin C / CST3 Protein (His tag); Aa1249; Ab1-341; Ab2-196; Ac1-114; Ac1262; Ac2-069; Ba2-693; CST3 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM HEPES, 150mM NaCl, pH 7.0
• Sequence Length: 140

• Application Notes: The recombinant rat CST3 comprises 131 amino acids and predicts a molecular mass of 14.7 kDa. The apparent molecular mass of the rat CST3 is approximately 18-25 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Gly 21
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CST3 recombinant protein

Background: Cystatin C, also known as Cystatin-3 (CST3) is a secreted type 2 cysteine protease inhibitor synthesized in all nucleated cells, has been proposed as a replacement for serum creatinine for the assessment of renal function, particularly to detect small reductions in glomerular filtration rate. The mature, active form of human cystatin C is a single non-glycosylated polypeptide chain consisting of 120 amino acid residues, with a molecular mass of 13, 343-13, 359 Da, and containing four characteristic disulfide-paired cysteine residues. Cystatin C is a low-molecular-weight protein which has been proposed as a marker of renal function that could replace creatinine. Indeed, the concentration of Cystatin C is mainly determined by glomerular filtration and is particularly of interest in clinical settings where the relationship between creatinine production and muscle mass impairs the clinical performance of creatinine. Since the last decade, numerous studies have evaluated its potential use in measuring renal function in various populations. More recently, other potential developments for its clinical use have emerged. In almost all the clinical studies, Cystatin C demonstrated a better accuracy than serum creatinine in discriminating normal from impaired kidney function, but controversial results have been obtained by comparing this protein with other indices of kidney disease, especially serum creatinine-based equations, such as early atherosclerosis, Alzheimer's dementia, vascular aneurysms, hyperhomocysteinaemia and other neurodegenerative diseases. Cystatin C could be a useful clinical tool to identify HIV-infected persons. In addition, its expression is up-regulated in malignance of certain tumor progression.

Description: A DNA sequence encoding the rat CST3 (P14841-1) (Met 1-Ala 140) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 54234046
• NCBI GeneID: 25307
• NCBI Accession #: NP_036969.1
• NCBI GenBank Nucleotide #: NM_012837.1
• UniProt Accession #: P14841; Q5M968
• Molecular Weight: 15,437 Da
• NCBI Official Full Name: cystatin-C
• NCBI Official Synonym Full Names: cystatin C
• NCBI Official Symbol: Cst3
• NCBI Official Synonym Symbols: CYSC
• NCBI Protein Information: cystatin-C
• UniProt Protein Name: Cystatin-C
• Protein Family: Cystatin
• UniProt Gene Name: Cst3

NCBI Description

extracellular cysteine protease inhibitor that may be involved in amyloidosis [RGD, Feb 2006]

Uniprot Description

CST3: As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Defects in CST3 are the cause of amyloidosis type 6 (AMYL6); also known as hereditary cerebral hemorrhage with amyloidosis (HCHWA), cerebral amyloid angiopathy (CAA) or cerebroarterial amyloidosis Icelandic type. AMYL6 is a hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low. Genetic variations in CST3 are associated with age- related macular degeneration type 11 (ARMD11). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Belongs to the cystatin family.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q41

Cellular Component: axon; basement membrane; cell projection; cell soma; contractile fiber; cytoplasm; endoplasmic reticulum; extracellular region; extracellular space; lysosome; multivesicular body; nuclear membrane; perinuclear region of cytoplasm; vesicle

Molecular Function: beta-amyloid binding; cysteine protease inhibitor activity; endopeptidase inhibitor activity; identical protein binding; protease binding; protease inhibitor activity

Biological Process: apoptosis; brain development; cell activation; circadian sleep/wake cycle, REM sleep; defense response; embryo implantation; eye development; fibril organization and biogenesis; male gonad development; negative regulation of peptidase activity; negative regulation of proteolysis; positive regulation of cell proliferation; positive regulation of DNA replication; regulation of programmed cell death; regulation of tissue remodeling; response to axon injury; response to carbohydrate stimulus; response to drug; response to estradiol stimulus; response to hypoxia; response to inorganic substance; response to nutrient levels; response to organic cyclic substance; response to oxidative stress; response to toxin; salivary gland development; Sertoli cell development