Uchl3 active protein

Uchl3, 1-230aa, Mouse, His tag, Baculovirus (Bioactivity Validated)

Cat. Num. AAA206419

• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: Uchl3; 1-230aa; Mouse; His tag; Baculovirus (Bioactivity Validated); Ubiquitin carboxyl-terminal hydrolase isozyme L3; Ubiquitin carboxyl-terminal esterase L3; Ubiquitin thioesterase L3; Ubiquitin thiolesterase; Ubiquitin thiolesterase L3; UCH L3; UCH-L3; UCHL3; UCHL3_HUMAN; Uchl3 active protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMEPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERAKFLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGKTSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA LEHHHHHH
• Sequence Length: 230

• Applicable Applications for Uchl3 active protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Biological Activity: Specific activity is > 9,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5, at 37C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Uchl3 active protein

Uchl3, as known as ubiquitin carboxyl-terminal hydrolase isozyme L3, is a member of the peptidase C12 family of deubiquitinating enzymes. It is widely expressed with the highest levels being observed in heart, testis, thymus and striated muscle. This protein is suggested to function in the central nervous system. In particular, it is involved in the maintenance of neurons of the gracile tract, nucleus tractus, solitaris, and area postrema, and in working memory. Recombinant mouse Uchl3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for Uchl3 active protein

Ubiquitination

References

Mtango NR., et al. (2012) J. Cell. Physiol. 227:2022-2029.; Dennissen FJ., et al. (2011) FEBS Lett. 585:2568-2574.

NCBI and Uniprot Product Information

• NCBI GI #: 139948802
• NCBI GeneID: 50933
• NCBI Accession #: NP_057932
• NCBI GenBank Nucleotide #: NM_016723.2
• UniProt Accession #: Q9JKB1; Q9EQX7
• Molecular Weight: 27.2 kDa (238aa) 28-40kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L3
• NCBI Official Synonym Full Names: ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
• NCBI Official Symbol: Uchl3
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L3
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L3
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: Uchl3
• UniProt Synonym Gene Names: UCH-L3

Uniprot Description

Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome.