Cathepsin X/Z/P Active Protein | CTSZ active protein

Recombinant Cathepsin X/Z/P

Cat. Num. AAA201113

• Gene Names: CTSZ; CTSX
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: Cathepsin X/Z/P; Recombinant Cathepsin X/Z/P; Cathepsin Z; CTSZ; CTSX; CTSP; CTSZ active protein

• Host: Baculovirus
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 10% Glycerol.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 24-303aa
• Sequence: GLYFRRGQTC YRPLRGDGLA PLGRSTYPRP HEYLSPADLP KSWDWRNVDG VNYASITRNQ HIPQYCGSCW AHASTSAMAD RINIKRKGAW PSTLLSVQNV IDCGNAGSCE GGNDLSVWDY AHQHGIPDET CNNYQAKDQE CDKFNQCGTC NEFKECHAIR NYTLWRVGDY GSLSGREKMM AEIYANGPIS CGIMATERLA NYTGGIYAEY QDTTYINHVV SVAGWGISDG TEYWIVRNSW GEPWGERGWL RIVTSTYKDG KGARYNLAIE EHCTFGDPIV LEHHHHHH
• Sequence Length: 303

• Applicable Applications for CTSZ active protein: Enzyme Activity, SDS-PAGE
• Species: Human
• Endotoxin: <1.0 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Predicted N-Terminal: Gly 24
• Biological Activity: Specific activity is > 1,400 pmol/min/ug in which one unit will convert 1.0 pmole of Mca-PLGL-Dpa-AR-NH2 to MCA- Pro-Leu-OH per minute at pH 3.5 at 25C.
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

Related Product Information for CTSZ active protein

Cathepsin Z, also known Cathepsin X and Cathepsin P, is a member of the C1 family of lysosomal cysteine proteases. It exhibits carboxy-monopeptidase and carboxy-dipeptidase activity. This protein regulates maturation of dendritic cells and plays important role in the initiation of adaptive immunity. It is also involved in tumor and immune cells as higher expression is found in tumour and immune cells of prostate and gastric carcinomas and inmacrophages of gastric mucosa. Recombinant human Cathepsin Z protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for CTSZ active protein

Cancer

References

Akkari L., et al, (2014) Genes Dev. 28:2134-2150. Allan ERO., et al, (2017) J Neuroinflammation. 14:103.

NCBI and Uniprot Product Information

• NCBI GI #: 22538442
• NCBI GeneID: 1522
• NCBI Accession #: NP_001327.2
• NCBI GenBank Nucleotide #: NP_001327.2
• UniProt Accession #: Q9UBR2
• Molecular Weight: 32.5kDa (288aa); 28-40KDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: cathepsin Z preproprotein
• NCBI Official Synonym Full Names: cathepsin Z
• NCBI Official Symbol: CTSZ
• NCBI Official Synonym Symbols: CTSX
• NCBI Protein Information: cathepsin Z
• UniProt Protein Name: Cathepsin Z
• Protein Family: Cathepsin
• UniProt Gene Name: CTSZ
• UniProt Entry Name: CATZ_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal cysteine proteinase and member of the peptidase C1 family. It exhibits both carboxy-monopeptidase and carboxy-dipeptidase activities. The encoded protein has also been known as cathepsin X and cathepsin P. This gene is expressed ubiquitously in cancer cell lines and primary tumors and, like other members of this family, may be involved in tumorigenesis. [provided by RefSeq, Oct 2008]

Uniprot Description

CTSZ: Exhibits carboxy-monopeptidase as well as carboxy- dipeptidase activity. Belongs to the peptidase C1 family.

Protein type: EC 3.4.18.1; Protease

Chromosomal Location of Human Ortholog: 20q13.32

Cellular Component: extracellular space; intracellular membrane-bound organelle; lysosome; endoplasmic reticulum; plasma membrane

Molecular Function: cysteine-type peptidase activity

Biological Process: cellular protein metabolic process; proteolysis; angiotensin maturation

Disease: Mycobacterium Tuberculosis, Susceptibility To