Ferritin heavy chain 1/FTH1 Recombinant Protein | FTH1 recombinant protein

Ferritin heavy chain 1/FTH1 Protein, Human, Recombinant

Cat. Num. AAA8121333

• Gene Names: FTH1; FHC; FTH; PLIF; FTHL6; PIG15
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Ferritin heavy chain 1/FTH1; Ferritin heavy chain 1/FTH1 Protein; Human; Recombinant; Human FTH1 Protein; FHC Protein; FTH Protein; FTHL6 Protein; HFE5 Protein; PIG15 Protein; PLIF Protein; ferritin; heavy polypeptide 1; FTH1 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser183

• Species: Human
• Predicted N Terminal: Met 1
• Tag: Native
• Protein Construction: A DNA sequence encoding the human FTH1 (P02794)(Met 1-Ser183) was expressed and purified.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for FTH1 recombinant protein

Background: FTH1 (ferritin, heavy polypeptide 1) is the heavy subunit of ferritin which is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. FTH1 gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. FTH1 stores iron in a soluble, non-toxic, readily available form. It is important for iron homeostasis. It has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. It also plays a role in delivery of iron to cells. FTH1 mediates iron uptake in capsule cells of the developing kidney.

References

Hentze MW, et al. (1986) Cloning, characterization, expression, and chromosomal localization of a human ferritin heavy-chain gene. Proc Natl Acad Sci. 83(19):7226-30.Rual, et al. (2005) Towards a proteome-scale map of the human protein-protein interaction network. Nature. 437(7062):1173-8.Stelzl, et al. (2005) A human protein-protein interaction network: a resource for annotating the proteome. Cell. 122(6):957-968.

NCBI and Uniprot Product Information

• NCBI GI #: 56682959
• NCBI GeneID: 2495
• NCBI Accession #: NP_002023.2
• NCBI GenBank Nucleotide #: NM_002032.2
• UniProt Accession #: P02794; Q3KRA8; Q3SWW1; B3KNR5
• Molecular Weight: 21,226 Da
• NCBI Official Full Name: ferritin heavy chain
• NCBI Official Synonym Full Names: ferritin, heavy polypeptide 1
• NCBI Official Symbol: FTH1
• NCBI Official Synonym Symbols: FHC; FTH; PLIF; FTHL6; PIG15
• NCBI Protein Information: ferritin heavy chain; apoferritin; ferritin H subunit; placenta immunoregulatory factor; proliferation-inducing protein 15; cell proliferation-inducing gene 15 protein
• UniProt Protein Name: Ferritin heavy chain
• Protein Family: Iron transporter
• UniProt Gene Name: FTH1
• UniProt Synonym Gene Names: FTH; FTHL6; Ferritin H subunit
• UniProt Entry Name: FRIH_HUMAN

NCBI Description

This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Ferritin-H: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited. Belongs to the ferritin family.

Protein type: Oxidoreductase; EC 1.16.3.1; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: ferritin complex; mitochondrion; cytosol; nucleus

Molecular Function: ferroxidase activity; protein binding; ferric iron binding; iron ion binding

Biological Process: receptor-mediated endocytosis; negative regulation of cell proliferation; cellular iron ion homeostasis; negative regulation of fibroblast proliferation; immune response; post-Golgi vesicle-mediated transport; iron ion transport; transmembrane transport; intracellular sequestering of iron ion

Disease: Hemochromatosis, Type 5