Typical Testing Data/Standard Curve (for reference only)
Trypsin Assay Kit | TRYP assay kit
Trypsin Activity Colorimetric Assay Kit
Synonyms
Trypsin; Trypsin Activity Colorimetric Assay Kit; TRYP assay kit
Sequence Length
248
Samples
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, etc.
Features & Benefits
Simple procedure; takes ~ 1-2 hours based on the levels of trypsin
Fast and convenient
Fast and convenient
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TRYP assay kit
Product Information: Trypsin Activity Assay Kit: Colorimetric Assay for detecting Trypsin Activity in Tissues, Cells, Serum etc. Rapid, Convenient & Sensitive. Detection Range: 10 - 100 mU. 100 Assays.
Background: Trypsin (EC 3.4.21.4) is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins. Trypsin is produced in the pancreas as the inactive proenzyme trypsinogen. Active trypsin predominantly cleaves peptide chains at the carboxyl side of the amino acids lysine or arginine, except when either is followed by proline. It is used for numerous biotechnological processes. In the assay, trypsin cleaves a substrate to generate p-nitroaniline (p-NA) which is detected at lambda=405 nm. Since the color intensity is proportional to p-NA content, trypsin activity can be accurately measured. The kit detects 10-100 mU (p-NA unit) trypsin in various samples.
Summary: Detection method- Absorbance (405 nm)
Species reactivity- Mammalian
Application- The kit detects 10-100 mU (p-NA unit) trypsin in various samples.
Background: Trypsin (EC 3.4.21.4) is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins. Trypsin is produced in the pancreas as the inactive proenzyme trypsinogen. Active trypsin predominantly cleaves peptide chains at the carboxyl side of the amino acids lysine or arginine, except when either is followed by proline. It is used for numerous biotechnological processes. In the assay, trypsin cleaves a substrate to generate p-nitroaniline (p-NA) which is detected at lambda=405 nm. Since the color intensity is proportional to p-NA content, trypsin activity can be accurately measured. The kit detects 10-100 mU (p-NA unit) trypsin in various samples.
Summary: Detection method- Absorbance (405 nm)
Species reactivity- Mammalian
Application- The kit detects 10-100 mU (p-NA unit) trypsin in various samples.
Product Categories/Family for TRYP assay kit
NCBI and Uniprot Product Information
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Product Notes
The Trypsin tryp (Catalog #AAA841816) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Trypsin, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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