Testing Data
Free Fatty Acid Assay Kit
Free Fatty Acid Assay Kit (Colorimetric)
Synonyms
Free Fatty Acid; Free Fatty Acid Assay Kit (Colorimetric); Free Fatty Acid assay kit
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store the entire kit at -80 degree C. Avoid multiple freeze/thaws by aliquoting. The NEM Reagent and Colorimetric Probe are light sensitive and should be maintained in amber tubes.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for Free Fatty Acid assay kit
Background: Triglycerides (TAG) are a type of lipid in the blood, serving as an energy source and playing a key role in metabolism. Triglycerides are the digestive end product of breaking down dietary fats. Any extra carbohydrates and fats that are not immediately used are chemically converted into triglycerides. In the intestines, secreted enzyme lipases hydrolyze the triglyceride ester bond, yielding glycerol and free fatty acids (FFA) in a process called lipolysis. Additionally, hormones induce and regulate lipase activity in adipose tissue, resulting in changes to blood FFA levels. Free fatty acids then bind plasma albumin for circulation in the body, serving as a readily absorbed energy source for muscle, brain and other organ tissues. Measurement of free fatty acids has become useful in monitoring and identification of several diseases and metabolic disorders (e.g. obesity, insulin resistance, diabetes, cancer). Free Fatty Acid Assay Kit measures non-esterified fatty acids (NEFA) in serum and plasma by a coupled enzymatic reaction system (ACS-ACOD Method). First, Acyl CoA Synthetase (ACS) catalyzes fatty acid acylation of coenzyme A. Next, the acyl-CoA product is oxidized by Acyl CoA Oxidase (ACOD), producing hydrogen peroxide which reacts with the kit's Colorimetric Probe (absorbance maxima of 570 nm). The Free Fatty Acid Assay Kit is a simple, colorimetric assay that quantitatively measures the free fatty acid concentration (non-esterified) in various samples using a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, standards and unknown samples. The kit contains a palmitic acid standard and has a detection sensitivity limit of ~15 muM.
Product Categories/Family for Free Fatty Acid assay kit
References
1. Boden, G., Cheung, P., Stein, T.P. et al. (2002) Am J. Physiol. 283, E12-E-19.
2. Steinberg, H.O., Tarshoby, M., Monestel, R. et al. (1997) J. Clin. Invest. 100, 1230-1239.
3. Boden, G., Lebed, B. Schatz, M. et al. (2001) Diabetes 50, 1612-1617.
4. Boden, G., She, P., Mozzoli, M. et al. (2005) Diabetes 54, 917-927.
5. Kelley, D.E., Mokan, M., Simoneau, J.A., and Mandarino, L.J. (1993) J. Clin. Invest. 92, 91-98.
6. Lewis, G.F., Uffelman, K.D., Szeto, L.W., Weller, B., and Steiner, G. (1995) J. Clin. Invest. 95, 158-166.
Diané, A. et al. (2014). PACAP is essential for the adaptive thermogenic response of brown adipose tissue to cold exposure. J Endocrinol. 222:327-339.
2. Steinberg, H.O., Tarshoby, M., Monestel, R. et al. (1997) J. Clin. Invest. 100, 1230-1239.
3. Boden, G., Lebed, B. Schatz, M. et al. (2001) Diabetes 50, 1612-1617.
4. Boden, G., She, P., Mozzoli, M. et al. (2005) Diabetes 54, 917-927.
5. Kelley, D.E., Mokan, M., Simoneau, J.A., and Mandarino, L.J. (1993) J. Clin. Invest. 92, 91-98.
6. Lewis, G.F., Uffelman, K.D., Szeto, L.W., Weller, B., and Steiner, G. (1995) J. Clin. Invest. 95, 158-166.
Diané, A. et al. (2014). PACAP is essential for the adaptive thermogenic response of brown adipose tissue to cold exposure. J Endocrinol. 222:327-339.
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Product Notes
The Free Fatty Acid (Catalog #AAA168010) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Free Fatty Acid, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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