Integrase (Wild-Type) Assay Kit
Integrase Assay Kit (Wild-Type)
Synonyms
Integrase (Wild-Type); Integrase Assay Kit (Wild-Type); Integrase (Wild-Type) assay kit
Sequence Length
357
Preparation and Storage
-70C
Related Product Information for Integrase (Wild-Type) assay kit
Background/Introduction: HIV DNA is integrated into host DNA by a viral encoded integrase and this enzyme activity is a likely target site in the HIV life cycle to block viral infection. The HIV-1 Integrase Assay Kit is a non-radioactive assay used to quantitatively measure integrase activity, the effects of interacting proteins, anti-viral compounds, and other test articles on HIV-1 integrase activity. Streptavidin-coated 96-well plates are coated with a double-stranded HIV-1 LTR U5 donor substrate (DS) DNA containing an end-labeled biotin. Full-length recombinant HIV-1 integrase protein is loaded onto the DS DNA substrate. Integrase test articles are added to the enzyme reaction and then a different doublestranded target substrate (TS) DNA containing a 3'-end modification is added to the reaction mixture. The HIV-1 integrase cleaves the terminal two bases from the exposed 3'-end of the HIV-1 LTR DS DNA and then catalyzes a strand-transfer recombination reaction to integrate the DS DNA into the TS DNA. The products of the reaction are detected colorimetrically using an HRP-labeled antibody directed against the TS 3'-end modification. Sodium azide is included in the kit as a positive control compound that inhibits HIV-1 integrase catalytic activity.
Product Categories/Family for Integrase (Wild-Type) assay kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
integrase
Similar Products
Product Notes
The Integrase (Wild-Type) (Catalog #AAA412318) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Integrase (Wild-Type), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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