Actin Polyclonal Antibody | anti-ACTA1 antibody

Anti-Actin Antibody

Cat. Num. AAA178062

• Gene Names: ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: Actin; Polyclonal Antibody; Anti-Actin Antibody; ACTA; ACTA1; Actin alpha skeletal muscle; actin; alpha 1; skeletal muscle 1; skeletal muscle; alpha skeletal muscle; actina; actine; ACTS_HUMAN; aktin; Alpha Actin 1; Alpha skeletal muscle Actin; alpha-actin; Alpha-actin-1; ASMA; CFTD; CFTD1antibody; CFTDM; MPFD; NEM1; NEM2; NEM3; nemaline myopathy type 3; anti-ACTA1 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 377

• Applicable Applications for anti-ACTA1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Actin (277-308aa HETTYNSIMKCDIDIRKDLYANNVMSGGTTMY), identical to the related mouse and rat sequences.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- Actin Picoband antibody, MBS178062, Western blottingAll lanes: Anti Actin (MBS178062) at 0.5ug/mlLane 1: Rat Spleen Tissue Lysate at 50ugLane 2: Mouse Spleen Tissue Lysate at 50ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 42KDObserved bind size: 42KD )

Immunohistochemistry (IHC)

(Anti- Actin Picoband antibody, MBS178062,IHC(P)IHC(P): Mouse Spleen Tissue)

Immunohistochemistry (IHC)

(Anti- Actin Picoband antibody, MBS178062,IHC(P)IHC(P): Rat Spleen Tissue)

Immunohistochemistry (IHC)

(Anti- Actin Picoband antibody, MBS178062,IHC(P)IHC(P): Human Mammary Cancer Tissue)

Related Product Information for anti-ACTA1 antibody

Description: Rabbit IgG polyclonal antibody for Actin, alpha skeletal muscle(ACTA1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

References

1. Gunning,P., Ponte,P., Okayama,H., Engel,J., Blau,H. and Kedes,L.Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.Mol. Cell. Biol.1983; 3 (5), 787-795. 2. Goebel,H.H., Brockmann,K., Bonnemann,C.G., Warlo,I.A., Hanefeld,F.,Labeit,S., Durling,H.J. and Laing,N.G. Actin- related myopathy without any missense mutation in the ACTA1 Gene.J. Child Neurol.2004; 19 (2), 149-153. 3. Laing,N.G., Clarke,N.F., Dye,D.E., Liyanage,K., Walker,K.R.,Kobayashi,Y., Shimakawa,S., Hagiwara,T., Ouvrier,R., Sparrow,J.C., Nishino,I., North,K.N. and Nonaka,I.Actin mutations are one cause of congenital fibre type disproportion.Ann. Neurol.2004; 56 (5), 689-694.

NCBI and Uniprot Product Information

• NCBI GI #: 4501881
• NCBI GeneID: 58
• NCBI Accession #: NP_001091.1
• NCBI GenBank Nucleotide #: NM_001100.3
• UniProt Accession #: P68133; P02568; P99020; Q5T8M9
• Molecular Weight: 42,051 Da
• NCBI Official Full Name: actin, alpha skeletal muscle
• NCBI Official Synonym Full Names: actin, alpha 1, skeletal muscle
• NCBI Official Symbol: ACTA1
• NCBI Official Synonym Symbols: ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM
• NCBI Protein Information: actin, alpha skeletal muscle
• UniProt Protein Name: Actin, alpha skeletal muscle
• Protein Family: Actin
• UniProt Gene Name: ACTA1
• UniProt Synonym Gene Names: ACTA
• UniProt Entry Name: ACTS_HUMAN

NCBI Description

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]

Uniprot Description

ACTA1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells. Belongs to the actin family.

Protein type: Motility/polarity/chemotaxis; Contractile; Cell development/differentiation; Cytoskeletal

Chromosomal Location of Human Ortholog: 1q42.13

Cellular Component: actin cytoskeleton; actin filament; cytosol; extracellular space; filopodium; lamellipodium; sarcomere; stress fiber; striated muscle thin filament

Molecular Function: ADP binding; ATP binding; myosin binding; protein binding; structural constituent of cytoskeleton

Biological Process: cell growth; muscle contraction; muscle filament sliding; muscle thin filament assembly; response to extracellular stimulus; response to lithium ion; response to mechanical stimulus; response to steroid hormone stimulus; skeletal muscle fiber adaptation; skeletal muscle fiber development

Disease: Myopathy, Congenital, With Fiber-type Disproportion; Nemaline Myopathy 3