Activin A Receptor Type II A (ACVR2A) Recombinant Protein | ACVR2A recombinant protein

Recombinant Activin A Receptor Type II A (ACVR2A)

Cat. Num. AAA2009906

• Gene Names: ACVR2A; ACVR2; ACTRII
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Activin A Receptor Type II A (ACVR2A); Recombinant Activin A Receptor Type II A (ACVR2A); ACVR2A recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-A ILGRSETQEC LFFNANWEKD RTNQTGVEPC YGDKDKRRHCFATWKNISGS IEIVKQGCWLDDINCYDRTD CVEKKDSPEV YFCCCEGNMC NEKFSYFPEMEVTQPTSNPV TPKPP
• Sequence Length: 513

• Applicable Applications for ACVR2A recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ala20~Pro135 (Accession # P27037) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 518828583
• NCBI GeneID: 92
• NCBI Accession #: NP_001265508.1
• NCBI GenBank Nucleotide #: NM_001278579.1
• UniProt Accession #: P27037; Q53TH4; Q6NWV2; Q92474; B2RAB8; D3DP85
• Molecular Weight: 40.5kDa
• NCBI Official Full Name: activin receptor type-2A isoform 1
• NCBI Official Synonym Full Names: activin A receptor, type IIA
• NCBI Official Symbol: ACVR2A
• NCBI Official Synonym Symbols: ACVR2; ACTRII
• NCBI Protein Information: activin receptor type-2A
• UniProt Protein Name: Activin receptor type-2A
• Protein Family: Activin receptor
• UniProt Gene Name: ACVR2A
• UniProt Synonym Gene Names: ACVR2; ACTR-IIA; ACTRIIA
• UniProt Entry Name: AVR2A_HUMAN

NCBI Description

This gene encodes a receptor that mediates the functions of activins, which are members of the transforming growth factor-beta (TGF-beta) superfamily involved in diverse biological processes. The encoded protein is a transmembrane serine-threonine kinase receptor which mediates signaling by forming heterodimeric complexes with various combinations of type I and type II receptors and ligands in a cell-specific manner. The encoded type II receptor is primarily involved in ligand-binding and includes an extracellular ligand-binding domain, a transmembrane domain and a cytoplasmic serine-threonine kinase domain. This gene may be associated with susceptibility to preeclampsia, a pregnancy-related disease which can result in maternal and fetal morbidity and mortality. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

ACVR2A: a tyrosine-kinase like receptor kinase of the STKR family. The holoreceptor receptor is a heteromeric complex of receptor serine kinases which include at least two type I (I and IB) and two type II (II and IIB) receptors. Each is composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic kinase domain. Type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptor kinases are apparently constitutively active.

Protein type: Membrane protein, integral; Protein kinase, TKL; EC 2.7.11.30; Protein kinase, Ser/Thr (receptor); Kinase, protein; Motility/polarity/chemotaxis; TKL group; STKR family; Type2 subfamily

Chromosomal Location of Human Ortholog: 2q22.3

Cellular Component: cell surface; integral to plasma membrane; cytoplasm; plasma membrane; receptor complex

Molecular Function: transforming growth factor beta receptor activity; protein serine/threonine kinase activity; protein binding; activin receptor activity; protein self-association; growth factor binding; metal ion binding; activin binding; coreceptor activity; transmembrane receptor protein serine/threonine kinase activity; ATP binding; PDZ domain binding; receptor signaling protein serine/threonine kinase activity

Biological Process: embryonic skeletal development; positive regulation of erythrocyte differentiation; regulation of BMP signaling pathway; activin receptor signaling pathway; Sertoli cell proliferation; gastrulation with mouth forming second; positive regulation of bone mineralization; protein amino acid phosphorylation; anterior/posterior pattern formation; BMP signaling pathway; positive regulation of osteoblast differentiation; positive regulation of activin receptor signaling pathway; transmembrane receptor protein serine/threonine kinase signaling pathway; mesoderm development; spermatogenesis; sperm ejaculation; regulation of nitric-oxide synthase activity; positive regulation of protein amino acid phosphorylation; determination of left/right symmetry; penile erection