Rabbit HSPA5 Polyclonal Antibody | anti-HSPA5 antibody

HSPA5, ID (HSPA5, GRP78, 78kD glucose-regulated protein, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Heat shock 70kD protein 5, Immunoglobulin heavy chain-binding protein)

Cat. Num. AAA646230

• Gene Names: HSPA5; BIP; MIF2; GRP78
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: HSPA5; Polyclonal Antibody; ID (HSPA5; GRP78; 78kD glucose-regulated protein; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70kD protein 5; Immunoglobulin heavy chain-binding protein); Anti -HSPA5; anti-HSPA5 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: H,Z
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-HSPA5 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Immunofluorescence: 1:10-50
• Immunogen: HSPA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-300 amino acids from the Central region of human HSPA5.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HSPA5 Antibody (Center) western blot analysis in Hela,A2058,NCI-H460 cell line lysates (35ug/lane).This demonstrates the HSPA5 antibody detected the HSPA5 protein (arrow).)

Immunohistochemistry (IHC)

(HSPA5 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSPA5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(HSPA5 Antibody (Center) western blot analysis in zebra fish brain tissue lysates (35ug/lane).This demonstrates the HSPA5 antibody detected the HSPA5 protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HSPA5 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-HSPA5 antibody

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.

Product Categories/Family for anti-HSPA5 antibody

Antibodies; Abs to Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 86577744
• NCBI GeneID: 3309
• UniProt Accession #: P11021; Q2EF78; Q9NPF1; Q9UK02; B0QZ61
• Molecular Weight: 72,333 Da
• NCBI Official Full Name: HSPA5 protein
• NCBI Official Synonym Full Names: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• NCBI Official Symbol: HSPA5
• NCBI Official Synonym Symbols: BIP; MIF2; GRP78
• NCBI Protein Information: 78 kDa glucose-regulated protein; immunoglobulin heavy chain-binding protein; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
• UniProt Protein Name: 78 kDa glucose-regulated protein
• UniProt Gene Name: HSPA5
• UniProt Synonym Gene Names: GRP78; GRP-78; BiP
• UniProt Entry Name: GRP78_HUMAN

NCBI Description

The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]

Uniprot Description

Function: Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. Ref.12 Ref.26

Subunit structure: Interacts with DNAJC1 (via J domain)

By similarity. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with TMEM132A and TRIM21. May form a complex with ERLEC1, OS9, SEL1L and SYVN1. Interacts with DNAJC10 and MX1. Interacts with METTL23. Ref.15 Ref.17 Ref.18 Ref.21 Ref.22 Ref.26

Subcellular location: Endoplasmic reticulum lumen. Melanosome. Cytoplasm

By similarity. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.19 Ref.20

Involvement in disease: Autoantigen in rheumatoid arthritis. Ref.10

Sequence similarities: Belongs to the heat shock protein 70 family.