Typical Testing Data/Standard Curve (for reference only)
Isoprostane Assay Kit
Isoprostane EIA Kit
Synonyms
Isoprostane; Isoprostane EIA Kit; 15-Isoprostane F2t ELISA Kit; Isoprostane assay kit
Assay Type
Competitive
Preparation and Storage
1. Store the components of this kit at the temperatures specified on the labels.
2. Unopened reagents are stable until the indicated kit expiration date.
3. For partial kit storage, all unused components should be stored at 4 degree C, and unused portions of the microplate should be returned to the foil pouch with a desiccant prior to being stored at 4 degree C.
2. Unopened reagents are stable until the indicated kit expiration date.
3. For partial kit storage, all unused components should be stored at 4 degree C, and unused portions of the microplate should be returned to the foil pouch with a desiccant prior to being stored at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Isoprostane assay kit
Principle of the Assay: This kit is a competitive enzyme-linked immunoassay (ELISA) for determining levels of 15-IsoP F2t in biological samples. Briefly, 15-IsoP F2t in the samples or standards competes with 15-IsoP F2t conjugated to horseradish peroxidase (HRP) for binding to a polyclonal antibody specific for 15- IsoP F2t coated on the microplate. The HRP activity results in color development when the substrate is added, with the intensity of the color proportional to the amount of 15-IsoP F2t bound and inversely proportional to the amount of unconjugated 15-IsoP F2t in the samples or standards.
Background/Introduction: Isoprostanes are prostaglandin-like compounds that are produced by free radical mediated peroxidation of lipoproteins. This kit is for the quantification of a representative isoprostane, 15- isoprostane F2t (also known as 8-epi-PGF2alpha, 8-iso-PGF2alpha or 8-isoprostane). Levels of 15-isoprostane F2t (15-IsoP F2t) in biological fluids have been shown to be useful for assessment of oxidant stress in vivo. 15-IsoP F2t has also been shown to be a potent vasoconstrictor in rat kidneys and rabbit lungs, and plays a causative role in atherogenesis. Elevated isoprostane levels are associated with hepatorenal syndrome, rheumatoid arthritis, atherosclerosis, and carcinogenesis. This kit can be used for the quantification of free 15-IsoP F2t in urine, plasma, serum, and tissue samples following Solid Phase Extraction (SPE) of the isoprostane-containing fraction. Instructions are also provided for the quantification of total 15-IsoP F2t following hydrolysis of phospholipids. NOTE: Alternatively, urine samples may be analyzed without SPE using a separate kit, MBS480410 Urinary Isoprostane ELISA also available.
Background/Introduction: Isoprostanes are prostaglandin-like compounds that are produced by free radical mediated peroxidation of lipoproteins. This kit is for the quantification of a representative isoprostane, 15- isoprostane F2t (also known as 8-epi-PGF2alpha, 8-iso-PGF2alpha or 8-isoprostane). Levels of 15-isoprostane F2t (15-IsoP F2t) in biological fluids have been shown to be useful for assessment of oxidant stress in vivo. 15-IsoP F2t has also been shown to be a potent vasoconstrictor in rat kidneys and rabbit lungs, and plays a causative role in atherogenesis. Elevated isoprostane levels are associated with hepatorenal syndrome, rheumatoid arthritis, atherosclerosis, and carcinogenesis. This kit can be used for the quantification of free 15-IsoP F2t in urine, plasma, serum, and tissue samples following Solid Phase Extraction (SPE) of the isoprostane-containing fraction. Instructions are also provided for the quantification of total 15-IsoP F2t following hydrolysis of phospholipids. NOTE: Alternatively, urine samples may be analyzed without SPE using a separate kit, MBS480410 Urinary Isoprostane ELISA also available.
References
1. Morrow, J., et. al.; (1990) Anal. Biochem. 14:1-10
2. Morrow, J. D., et. al.,; (1990) Proc. Natl. Acad. Sci. 87:9383-9387
3. Morrow, et. al.,; (1997) Prog. Lipid Res. 36:1-21
4. Morrow, J., et. al.; (1992) Proc. Natl. Acad. Sci. 89:10721-10725
5. Wang, et. al.; (1995) J. Pharm. Exp. Ther. 275:94-100
6. Morrow, J., et. al.; (1999) Anal. Biochem. 269:326-331
7. Roberts II, L. J., Morrow, J. D.; (2000) Free Radical Biol. Med. 28:505-513
8. Morrow, J. D., Roberts II, L. J.; Meth. Enz. 300: 3-12
2. Morrow, J. D., et. al.,; (1990) Proc. Natl. Acad. Sci. 87:9383-9387
3. Morrow, et. al.,; (1997) Prog. Lipid Res. 36:1-21
4. Morrow, J., et. al.; (1992) Proc. Natl. Acad. Sci. 89:10721-10725
5. Wang, et. al.; (1995) J. Pharm. Exp. Ther. 275:94-100
6. Morrow, J., et. al.; (1999) Anal. Biochem. 269:326-331
7. Roberts II, L. J., Morrow, J. D.; (2000) Free Radical Biol. Med. 28:505-513
8. Morrow, J. D., Roberts II, L. J.; Meth. Enz. 300: 3-12
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Product Notes
The Isoprostane (Catalog #AAA480409) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Isoprostane, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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