Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat 8-epi-prostaglandin F2alpha (8-epi-PGF2alpha) Standard Curve.)
Rat 8-epi-Prostaglandin F2alpha (8-epi-PGF2alpha) ELISA Kit | 8-epi-PGF2alpha elisa kit
Rat 8-epi-Prostaglandin F2alpha (8-epi-PGF2alpha) ELISA Kit
Reactivity
Rat
Synonyms
8-epi-Prostaglandin F2alpha (8-epi-PGF2alpha); Rat 8-epi-Prostaglandin F2alpha (8-epi-PGF2alpha) ELISA Kit; 8-epi-PGF2a; 8-epi-PGF2alpha elisa kit
Reactivity
Rat
Specificity
Rat 8-epi-prostaglandin F2alpha (8-epi-PGF2alpha) ELISA Kit has high sensitivity and excellent specificity for detection of Rat 8-epi-PGF2a. No significant cross-reactivity or interference between Rat 8-epi-PGF2a and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat 8-epi-prostaglandin F2alpha (8-epi-PGF2alpha) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat 8-epi-prostaglandin F2alpha (8-epi-PGF2alpha) Standard Curve.)
Related Product Information for 8-epi-PGF2alpha elisa kit
Description: This Rat 8-epi-prostaglandin F2alpha (8-epi-PGF2alpha) ELISA Kit employs a two-site sandwich ELISA to quantitate 8-epi-PGF2a in samples. An antibody specific for 8-epi-PGF2a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any 8-epi-PGF2a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 8-epi-PGF2a is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 8-epi-PGF2a bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissue phospholipids by oxygen radicals. Isoprostanes appear as artifacts in tissue and plasma samples which have undergone oxidative degradation during prolonged or improper storage. They also appear in the plasma and urine under normal conditions and are elevated by oxidative stress. At least one of the isoprostanes, 8-isoprostane (8-epi PGF2alpha), has been shown to have biological activity. It is a potent pulmonary and renal vasoconstrictor1 and has been implicated as a causative mediator of hepatorenal syndrome and pulmonary oxygen toxicity.2 8-Isoprostane has been proposed as a marker of antioxidant deficiency and oxidative stress and elevated levels have been found in heavy smokers.3 8-Isoprostane levels are also a relative indicator of sample integrity for lipid-containing samples such as serum, plasma, and whole cell preparations.
Background: The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissue phospholipids by oxygen radicals. Isoprostanes appear as artifacts in tissue and plasma samples which have undergone oxidative degradation during prolonged or improper storage. They also appear in the plasma and urine under normal conditions and are elevated by oxidative stress. At least one of the isoprostanes, 8-isoprostane (8-epi PGF2alpha), has been shown to have biological activity. It is a potent pulmonary and renal vasoconstrictor1 and has been implicated as a causative mediator of hepatorenal syndrome and pulmonary oxygen toxicity.2 8-Isoprostane has been proposed as a marker of antioxidant deficiency and oxidative stress and elevated levels have been found in heavy smokers.3 8-Isoprostane levels are also a relative indicator of sample integrity for lipid-containing samples such as serum, plasma, and whole cell preparations.
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Product Notes
The Rat 8-epi-PGF2alpha (Catalog #AAA9711936) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711936 ELISA Kit recognizes Rat 8-epi-PGF2alpha. It is sometimes possible for the material contained within the vial of "8-epi-Prostaglandin F2alpha (8-epi-PGF2alpha), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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