CytoSelect 96-well Phagocytosis Assay (Red Blood Cell) Assay Kit

CytoSelect 96-well Phagocytosis Assay (Red Blood Cell)

Cat. Num. AAA168645

• Synonyms: CytoSelect 96-well Phagocytosis Assay (Red Blood Cell); CytoSelect 96-well Phagocytosis Assay (Red Blood Cell) assay kit

• Preparation and Storage: Store all kit components at 4 degree C.

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Related Product Information for CytoSelect 96-well Phagocytosis Assay (Red Blood Cell) assay kit

Background: In mammals, phagocytosis by phagocytes (e.g.,macrophages, dendritic cells, and neutrophils) is essential for a variety of biological events, including tissue remodeling and the continuous clearance of dying cells. Furthermore, phagocytosis represents an early and crucial event in triggering host defenses against invading pathogens. Phagocytosis comprises a series of events, starting with the binding and recognition of particles by cell surface receptors, followed by the formation of actin-rich membrane extensions around the particle. Fusion of the membrane extensions results in phagosome formation, which precedes phagosome maturation into a phagolysosome. Pathogens inside the phagolysosome are destroyed by lowered pH, hydrolysis, and radical attack (Figure 1). These early events that are mediated by the innate immune system are critical for host survival. As a result of this process, pathogen-derived molecules can be presented at the cell surface (antigen presentation), allowing the induction of acquired immunity. Traditionally, erythrocytes (red blood cells) are commonly used in phagocytosis assay. For FcR mediated phagocytosis, erythrocytes are first opsonized with serum or IgG before they are added to phagocytes. After removal of non-phagocytic erythrocytes, engulfed erythrocytes per phagocyte cell are manually counted (expressed as phagocytosis index or engulfed erythrocytes per phagocyte). This manual counting method is quite cumbersome, time-consuming, and difficult when testing a large number of samples. CytoSelect 96-well Phagocytosis Assay does not involve subjective manual counting of erythrocytes. Instead cells are lyzed and detected by the proprietary erythrocyte substrate in a microtiter plate reader (Figure 2). This format provides a quantitative, high-throughput method to accurately measure phagocytosis. The CytoSelect 96-well Phagocytosis Assay provides a robust system for screening TLR ligands, phagocytosis activators or inhibitors. Each kit provides sufficient quantities to perform 96, 48, 24 tests in a 96, 48, 24-well plate, respectively.

Product Categories/Family for CytoSelect 96-well Phagocytosis Assay (Red Blood Cell) assay kit

Cell Based Assays; Phagocytosis Assays

References

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