Typical Testing Data/Standard Curve (for reference only)
Cortisol Assay Kit
Cortisol EIA Kit
Synonyms
Cortisol; Cortisol EIA Kit; Cortisol assay kit
Assay Type
Competitive
Preparation and Storage
1. Store the components of this kit at the temperatures specified on the labels.
2. Unopened reagents are stable until the indicated kit expiration date.
3. Desiccant bag must remain in foil pouch with unused strips. Keep pouch sealed when not in use to maintain a dry environment. Remove excess air before sealing.
2. Unopened reagents are stable until the indicated kit expiration date.
3. Desiccant bag must remain in foil pouch with unused strips. Keep pouch sealed when not in use to maintain a dry environment. Remove excess air before sealing.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Cortisol assay kit
Principle of the Assay: This kit is a competitive enzyme-linked immunoassay (ELISA) for determining levels of Cortisol in biological samples such as urine, saliva, and pl'csma. Briefly, cortisol in the samples or standards competes with cortisol conjugated to horseradish peroxidase (HRP) for binding to a polycJonal antibody specific for cortisol coated on the microplate. The HRP activity results in brilIiant blue color development when the substrate is added, with the intensity of the color proportional to the amount of cortisol-HRP bound and inversely proportional to the amount of unconjugated cortisol in the samples or standards.
Background: is produced throughout the day; with very high levels in the early morning it quickly decreases to a low level in the early afternoon. After that, it continues to slowly decrease into the night quickly spiking before morning. Cortisol levels may also spike immediately after waking up, during periods of low blood glucose levels, and in response to stress. Cortisol is also known as Hydrocortisone when it is used medicinally, usually as a supplement or for its anti- inflammatory properties. Knowing plasma Cortisol levels is useful in identifying a serious lack of cortisol usually found in Addison's disease, or a serious overproduction of cortisol as in Cushing's syndrome. Cortisol is also frequently used to evaluate the stress levels of a subject.
Background: is produced throughout the day; with very high levels in the early morning it quickly decreases to a low level in the early afternoon. After that, it continues to slowly decrease into the night quickly spiking before morning. Cortisol levels may also spike immediately after waking up, during periods of low blood glucose levels, and in response to stress. Cortisol is also known as Hydrocortisone when it is used medicinally, usually as a supplement or for its anti- inflammatory properties. Knowing plasma Cortisol levels is useful in identifying a serious lack of cortisol usually found in Addison's disease, or a serious overproduction of cortisol as in Cushing's syndrome. Cortisol is also frequently used to evaluate the stress levels of a subject.
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Product Notes
The Cortisol (Catalog #AAA480401) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Cortisol, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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