Human Cathepsin E ELISA Kit | CTSE elisa kit

Human Cathepsin E ELISA Kit

Cat. Num. AAA8244629

• Gene Names: CTSE; CATE
• Reactivity: Human
• Applications: ELISA
• Synonyms: Cathepsin E; Human Cathepsin E ELISA Kit; CTSE elisa kit

• Reactivity: Human
• Specificity: The Human Cathepsin E ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Cathepsin E proteins within the range of 156 pg/ml - 10000 pg/ml.
• Sequence Length: 401

• Applicable Applications for CTSE elisa kit: Sandwich ELISA (EIA)
• Samples: Cell Culture Supernatants, Serum, Plasma.
• Sensitivity: 10 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve

Related Product Information for CTSE elisa kit

Principle of the Assay||The Human Cathepsin E ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Cathepsin E in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human Cathepsin E coated on a 96-well plate. Standards and samples are pipetted into the wells and Cathepsin E present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Cathepsin E antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Cathepsin E bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Cathepsin E is a protein that in humans is encoded by the CTSE gene. It is mapped to 1q32.1. The protein encoded by this gene is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. Cathepsin E is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen, and it also has an important role in immune responses.

NCBI and Uniprot Product Information

• NCBI GI #: 46397366
• NCBI GeneID: 1510
• UniProt Accession #: P14091; Q5TZ01; Q5TZ02; Q9NY58; Q9UCE3; Q9UCE4
• Molecular Weight: 39,553 Da
• NCBI Official Full Name: Cathepsin E
• NCBI Official Synonym Full Names: cathepsin E
• NCBI Official Symbol: CTSE
• NCBI Official Synonym Symbols: CATE
• NCBI Protein Information: cathepsin E
• UniProt Protein Name: Cathepsin E
• Protein Family: Cathepsin
• UniProt Gene Name: CTSE
• UniProt Entry Name: CATE_HUMAN

NCBI Description

This gene encodes a member of the A1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme, an aspartic endopeptidase, may be involved in antigen processing and the maturation of secretory proteins. Elevated expression of this gene has been observed in neurodegeneration. [provided by RefSeq, Nov 2015]

Uniprot Description

CTSE: May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain. Belongs to the peptidase A1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.23.34; Protease

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: endosome

Molecular Function: aspartic-type endopeptidase activity; protein homodimerization activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; digestion; protein autoprocessing; protein catabolic process