Procathepsin E/Cathepsin E Active Protein | CTSE active protein

Procathepsin E / Cathepsin E, human recombinant

Cat. Num. AAA845148

• Gene Names: CTSE; CATE
• Applications: SDS-Page
• Purity: >=90%
• Synonyms: Procathepsin E/Cathepsin E; Procathepsin E / Cathepsin E; human recombinant; CTSE; Cathepsin E; Procathepsin E; CTSE active protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: Lyophilized from 5 mg/ml solution in a proprietary buffer; Appearance: Lyophilized
• Sequence Length: 396

• Applicable Applications for CTSE active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Reconstitute with water to 0.5-1 mg/ml. Aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.
• Activity (Specifications/test method): MyBioSource's Procathepsin E/ Cathepsin E has a specific activity of >500 mU/mg. The Procathepsin E is auto activated to form Cathepsin E after reconstitution. MyBioSource's Procathepsin E/ Cathepsin E protein is more stable than a mixture of these proteins made from a non-E.Coli source.
• Biological Activity: MyBioSource's Procathepsin E/ Cathepsin E has a specific activity of >500 mU/mg. The Procathepsin E is auto activated to form Cathepsin E after reconstitution. MyBioSource's Procathepsin E/ Cathepsin E protein is more stable than a mixture of these proteins made from a non-E.Coli source.
• Results: >500 mU/mg
• Unit Definition: 1 U = 1 nmole/min digestion of Cathepsin E fluorogenic substrate Mca-GKPILFFRLK(Dnp)-R-NH2 substrate in 50 mM sodium acetate, 0.5 M NaCl, pH 3.0 buffer.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for CTSE active protein

Background: Cathepsin E (EC: 3.4.23.34) (CTSE) is an intracellular gastric aspartyl protease. It was originally identified as a cathepsin D-like acid protease. It is active in acidic conditions in a pH range from 2.5 to 5.5. In vitro experiments have identified several CTSE substrates including insulin beta chain, neurokinin, and FGF. Although the function of CTSE is not completely understood, it has been implicated in several physiological and pathological processes. CTSE is required for antigen presentation on class II MHC molecules. CTSE-deficient macrophages show abnormalities such as autophagy. Like many other cathepsins, CTSE has emerged as a therapy target for cancers, such as pancreatic ductal adenocarcinoma (PDAC). In addition to PDAC, CTSE is also overexpressed in gastric carcinomas and cervical and lung adenocarcinomas. The possible involvement of CTSE in neurodegeneration has also been reported. This protease has a specificity similar to that of pepsin A and cathepsin D. It is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. It is found in more than half of gastric cancers.

Product Categories/Family for CTSE active protein

Apoptosis & Related Products; Non-Caspase Proteases; Cathepsin; Cathepsin EnzymesProteins and Enzymes; ProteasesProteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 4503145
• NCBI GeneID: 1510
• NCBI Accession #: NP_001901.1
• NCBI GenBank Nucleotide #: NM_001910.3
• UniProt Accession #: P14091; Q5TZ01; Q5TZ02; Q9NY58; Q9UCE3; Q9UCE4
• Molecular Weight: 43.6 kDa (20-401 aa + N-terminal polyhistidine tag)
• NCBI Official Full Name: cathepsin E isoform a preproprotein
• NCBI Official Synonym Full Names: cathepsin E
• NCBI Official Symbol: CTSE
• NCBI Official Synonym Symbols: CATE
• NCBI Protein Information: cathepsin E
• UniProt Protein Name: Cathepsin E
• Protein Family: Cathepsin
• UniProt Gene Name: CTSE
• UniProt Entry Name: CATE_HUMAN

NCBI Description

The protein encoded by this gene is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen. Transcript variants utilizing alternative polyadenylation signals and two transcript variants encoding different isoforms exist for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CTSE: May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain. Belongs to the peptidase A1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.23.34; Protease

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: endosome

Molecular Function: protein homodimerization activity; aspartic-type endopeptidase activity

Biological Process: protein autoprocessing; digestion; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis