Rabbit PRMT1 Polyclonal Antibody | anti-PRMT1 antibody

Anti-PRMT1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100699

• Gene Names: PRMT1; ANM1; HCP1; IR1B4; HRMT1L2
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: PRMT1; Polyclonal Antibody; Anti-PRMT1 Antibody; Rabbit Polyclonal; PRMT1 Antibody; Rabbit PAb; Antigen Affinity Purified; protein arginine methyltransferase 1; Anti-ANM1 Antibody; Anti-HCP1 Antibody; Anti-HRMT1L2 Antibody; Anti-IR1B4 Antibody; anti-PRMT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PRMT1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PRMT1 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:1000-1:10,000; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human PRMT1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human PRMT1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence staining of PRMT1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human PRMT1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus..)

Immunohistochemistry (IHC)

(Immunochemical staining PRMT1 in human prostatic carcinoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining PRMT1 in human breast carcinoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining PRMT1 in mouse brain with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining PRMT1 in rat brain with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-PRMT1 antibody

Protein arginine methyltransferase 1 (PRMT1) is a key player in the dynamic regulation of arginine methylation. Its dysregulation and aberrant expression are implicated in various pathological conditions, and a plethora of evidence suggests that PRMT1 inhibition is of significant therapeutic value.PRMT1 is the most highly expressed of the protein arginine methyltransferases, enzymes responsible for methylation of arginine motifs on histone and nonhistone proteins. PRMT1 regulates signal transduction and transcriptional activity that affect multiple signal pathways crucial in craniofacial development, such as the BMP, TGFbeta, and WNT pathways.

NCBI and Uniprot Product Information

• NCBI GI #: 161789011
• NCBI GeneID: 3276
• UniProt Accession #: Q99873; Q15529; Q2VP93; Q6LEU5; Q8WUW5; Q99872; Q99874; Q9NZ04; Q9NZ05; Q9NZ06; B4E3C3; G5E9B6
• Molecular Weight: 361
• NCBI Official Full Name: Protein arginine N-methyltransferase 1
• NCBI Official Synonym Full Names: protein arginine methyltransferase 1
• NCBI Official Symbol: PRMT1
• NCBI Official Synonym Symbols: ANM1; HCP1; IR1B4; HRMT1L2
• NCBI Protein Information: protein arginine N-methyltransferase 1; interferon receptor 1-bound protein 4; histone-arginine N-methyltransferase PRMT1; HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2; heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 2
• UniProt Protein Name: Protein arginine N-methyltransferase 1
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT1
• UniProt Synonym Gene Names: HMT2; HRMT1L2; IR1B4
• UniProt Entry Name: ANM1_HUMAN

NCBI Description

This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]

Uniprot Description

PRMT1: Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Homodimer and heterodimer with PRMT8. The dimer can then associate to form a homohexamer. Interacts with ILF3, BTG1, BTG2, SUPT5H and interferon-alpha/beta receptor 1. Interacts with NFATC2IP. Widely expressed. By BTG1, BTG2 and ILF3. Belongs to the protein arginine N-methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; EC 2.1.1.125; Nuclear receptor co-regulator; Methyltransferase, protein arginine

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: identical protein binding; protein-arginine omega-N asymmetric methyltransferase activity; histone methyltransferase activity; methyltransferase activity; protein binding; N-methyltransferase activity

Biological Process: histone methylation; peptidyl-arginine methylation; establishment and/or maintenance of chromatin architecture; cell surface receptor linked signal transduction; protein amino acid methylation; regulation of transcription, DNA-dependent; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; negative regulation of megakaryocyte differentiation; neurite development