Antioxidant Capacity (FRAP) Assay Kit | FRAP assay kit
Total Antioxidant Capacity Assay Kit (FRAP)
Synonyms
Antioxidant Capacity (FRAP); Total Antioxidant Capacity Assay Kit (FRAP); FRAP assay kit
Preparation and Storage
The kit is shipped on ice pack and storage at -20 degree C is valid in ~12 months, protected from light, is recommended.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for FRAP assay kit
Background/Introduction: Oxidants, such as reactive oxygen species (ROS) and reactive nitrogen species (RNS), can generate free radicals that can cause severe oxidative damage to cellular lipids, membranes, proteins, and DNA. Antioxidants can scavenge these free radicals and prevent cellular oxidative stress by enzymatic and non-enzymatic mechanisms. Enzyme systems that function as antioxidants include catalase and peroxidase. Tocopherols, carotenes, vitamin A, and ubiquinols function as lipid-soluble antioxidants; whereas, glutathione and ascorbate are some of the water-soluble antioxidants. Measurement of the total non-enzymatic antioxidant capacity (TAC) of biological samples is indicative of their ability to counteract oxidative stress-induced damage in cells. TAC is used to provide insights into the development and treatment of oxidative-stress related disorders. In the Total Antioxidant Capacity Assay Kit, either the concentration of the combination of both small molecule and protein antioxidants, or the concentration of only small molecule antioxidants can be determined. Under acidic condition, Fe3+- TPTZ is converted to Fe2+- TPTZ by both small molecules and proteins. The reduced Fe2+ion chelates with a colorimetric probe, giving a broad absorbance peak at 593 nm, which is proportional to the total antioxidant capacity. Because of acidic conditions, and the total plasma concentration of iron ion and ferrous ion in serum samples is usually lower than 10 uM, some endogenous interference factors can be inhibited.
