Asialoglycoprotein receptor 1 Recombinant Protein | ASGR1 recombinant protein

Recombinant Human Asialoglycoprotein receptor 1

Cat. Num. AAA1265368

• Gene Names: ASGR1; HL-1; ASGPR; ASGPR1; CLEC4H1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Asialoglycoprotein receptor 1; Recombinant Human Asialoglycoprotein receptor 1; C-type lectin domain family 4 member H1; Hepatic lectin H1; HL-1; ASGR1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Liquid containing glycerol
• Sequence Positions: 68-291aa; Partial
• Sequence: EELRGLRETFSNFTASTEAQVKGLSTQGGNVGRKMKSLESQLEKQQKDLSEDHSSLLLHVKQFVSDLRSLSCQMAALQGNGSERTCCPVNWVEHERSCYWFSRSGKAWADADNYCRLEDAHLVVVTSWEEQKFVQHHIGPVNTWMGLHDQNGPWKWVDGTDYETGFKNWRPEQPDDWYGHGLGGGEDCAHFTDDGRWNDDVCQRPYRWVCETELDKASQEPPLL
• Sequence Length: 291

• Production Note: Special Offer: The E Coli host-expressed protein is manufactured from a stock plasmid containing the protein gene. E Colihost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The E Coli host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select E Coli host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to [email protected] for more details.
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for ASGR1 recombinant protein

Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been roved. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

Product Categories/Family for ASGR1 recombinant protein

Signal Transduction

References

Sequence of human asialoglycoprotein receptor cDNA. An internal signal sequence for membrane insertion.Spiess M., Schwartz A.L., Lodish H.F.J. Biol. Chem. 260:1979-1982(1985) An internal signal sequence the asialoglycoprotein receptor membrane anchor.Spiess M., Lodish H.F.Cell 44:177-185(1986) Human asialoglycoprotein receptor 1 gene is expressed in SH-SY5Y human neuroblastoma cells.Wang H., Gao X., Li L., Lou H., Huang Y., Wang B., Han J.A new splice variant of the major subunit of human asialoglycoprotein receptor encodes a secreted form in hepatocytes.Liu J., Hu B., Yang Y., Ma Z., Yu Y., Liu S., Wang B., Zhao X., Lu M., Yang D.PLoS ONE 5:E12934-E12934(2010) DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L., Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.Nature 440:1045-1049(2006) Fatty acylation of the rat and human asialoglycoprotein receptors. A conserved cytoplasmic cysteine residue is acylated in all receptor subunits.Zeng F.Y., Weigel P.H.J. Biol. Chem. 271:32454-32460(1996) Cloning, mapping, and characterization of a human homologue of the yeast longevity assurance gene LAG1.Pan H., Qin W.-X., Huo K.-K., Wan D.-F., Yu Y., Xu Z.-G., Hu Q.-D., Gu K.T., Zhou X.-M., Jiang H.-Q., Zhang P.-P., Huang Y., Li Y.-Y., Gu J.-R.Genomics 77:58-64(2001) Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry.Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.J. Proteome Res. 8:651-661(2009) Initial characterization of the human central proteome.Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.BMC Syst. Biol. 5:17-17(2011) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.J. Proteomics 96:253-262(2014) Crystal structure of the carbohydrate recognition domain of the H1 subunit of the asialoglycoprotein receptor.Meier M., Bider M.D., Malashkevich V.N., Spiess M., Burkhard P.J. Mol. Biol. 300:857-865(2000)

NCBI and Uniprot Product Information

• NCBI GI #: 308387363
• NCBI GeneID: 432
• NCBI Accession #: NP_001184145.1
• NCBI GenBank Nucleotide #: NM_001197216.2
• UniProt Accession #: P07306; I3L1X1
• Molecular Weight: 29.7 kDa
• NCBI Official Full Name: asialoglycoprotein receptor 1 isoform b
• NCBI Official Synonym Full Names: asialoglycoprotein receptor 1
• NCBI Official Symbol: ASGR1
• NCBI Official Synonym Symbols: HL-1; ASGPR; ASGPR1; CLEC4H1
• NCBI Protein Information: asialoglycoprotein receptor 1
• UniProt Protein Name: Asialoglycoprotein receptor 1
• Protein Family: Asialoglycoprotein receptor
• UniProt Gene Name: ASGR1
• UniProt Synonym Gene Names: CLEC4H1; ASGP-R 1; ASGPR 1; HL-1
• UniProt Entry Name: ASGR1_HUMAN

NCBI Description

This gene encodes a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins with exposed terminal galactose or N-acetylgalactosamine residues. The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. The protein encoded by this gene is the more abundant major subunit. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

ASGR1: Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13.2

Cellular Component: extracellular region; integral to plasma membrane

Molecular Function: asialoglycoprotein receptor activity; carbohydrate binding; metal ion binding; protein binding; protein homodimerization activity

Biological Process: cellular response to extracellular stimulus; receptor-mediated endocytosis