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Western Blot (WB)
Rabbit RBBP4 Polyclonal Antibody | anti-RBBP4 antibody
Anti-RBBP4 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Synonyms
RBBP4; Polyclonal Antibody; Anti-RBBP4 Antibody; anti-RBBP4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Western blot analysis of extracts of various cell lines, using RBBP4 antibody. Immunohistochemistry of paraffin-embedded human lung cancer using RBBP4 antibody at dilution of 1:100 (400x lens). Immunohistochemistry of paraffin-embedded mouse kidney using RBBP4 antibody at dilution of 1:100 (400x lens). Immunohistochemistry of paraffin-embedded rat lung using RBBP4 antibody at dilution of 1:100 (200x lens). Immunofluorescence analysis of MCF7 cell using RBBP4 antibody. Blue: DAPI for nuclear staining.
Purity/Purification
Affinity Purification
Form/Format
Liquid
Concentration
1mg/ml (varies by lot)
Sequence Length
269
Applicable Applications for anti-RBBP4 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200
CHIP: 1:20 - 1:100
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200
CHIP: 1:20 - 1:100
Immunogen
Recombinant protein of human RBBP4
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.
Western Blot (WB)
Western Blot (WB)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Immunofluorescence (IF)
Immunofluorescence (IF)
Related Product Information for anti-RBBP4 antibody
Retinoblastoma-associated proteins 46 and 48 (RBAP46 and RBAP48; also known as RBBP7 and RBBP4) were first characterized in human cells as proteins that bind to the retinoblastoma (Rb) tumor suppressor protein (1). Since then, these proteins have been shown to be components of many protein complexes involved in chromatin regulation, including the chromatin assembly factor 1 (CAF-1) complex and type B histone acetyltransferase complex HAT1, both of which function in chromatin assembly during DNA replication (2, 3). RBAP46 and RBAP48 are also found in the nucleosome remodeling factor complex NURF, the nucleosome remodeling and histone de-acetylation complex NuRD, and the Sin3/HDAC histone de-acetylation complex (4-7). More recently, RBAP46 and RBAP48 were identified as components of the polycomb repressor complex PRC2, which also contains EED and Ezh2 (8). RBAP46 and RBAP48 bind to the histone fold region of histone H4 and are believed to target these chromatin remodeling, histone acetylation, and histone de-acetylation complexes to their histone substrates (3).
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
30,323 Da
NCBI Official Full Name
RBBP4
UniProt Protein Name
RBBP4
Protein Family
UniProt Gene Name
RBBP4
Similar Products
Product Notes
The RBBP4 rbbp4 (Catalog #AAA419674) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RBBP4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RBBP4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200 IP: 1:50 - 1:200 CHIP: 1:20 - 1:100. Researchers should empirically determine the suitability of the RBBP4 rbbp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RBBP4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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