Rabbit HtrA3 Polyclonal Antibody | anti-HtrA3 antibody

HtrA3 Antibody (C-term) Purified Rabbit Polyclonal Antibody (Pab)

Cat. Num. AAA9232369

• Gene Names: HTRA3; Prsp; Tasp
• Reactivity: Human; Predicted Reactivity: Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Synonyms: HtrA3; Polyclonal Antibody; HtrA3 Antibody (C-term) Purified Rabbit Polyclonal Antibody (Pab); Serine protease HTRA3; 3421-; High-temperature requirement factor A3; Pregnancy-related serine protease; HTRA3; PRSP; anti-HtrA3 antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Clone Number: RB2324
• Specificity: This HtrA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-425 amino acids from the C-terminal region of human HtrA3.
• Purity/Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

• Applicable Applications for anti-HtrA3 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA)
• Application Notes: WB: 1:1000; IHC-P: 1:50-100
• Antigen Source: Human
• Antigen Type: Synthetic Peptide
• Antigen Region: 394-425
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(WB: 1:1000Western blot analysis of anti-HtrA3 C-term Pab in mouse brain tissue lysate. HtrA3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)-Paraffin

(IHC-P: 1:50-100Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-HtrA3 antibody

Insulin-like growth factors (IGFs) stimulate the proliferation and differentiation of a vast number of cell types. The action of the growth factors is mediated and controlled by a complex system of components, including several proteases that cleave the IGF-Binding Proteins. HtrA1 is a 480 aa protein that contains an N-terminus homologous to MAC25 (IGFBP7) with a conserved Kazal-type serine protease inhibitor motif, as well as a C-terminal PDZ domain. Semiquantitative RT-PCR and immunoblot analyses showed an approximately 7-fold increase of PRSS11 in osteoarthritis cartilage compared with controls. HTRA2 is released from mitochondria and inhibits the function of XIAP by direct binding in a way similar to SMAC. Moreover, when overexpressed extramitochondrially, HTRA2 induced atypical cell death, which was neither accompanied by a significant increase in caspase activity nor inhibited by caspase inhibitors, including XIAP. A catalytically inactive mutant of HTRA2, however, did not induce cell death. Suzuki et al. (2001) concluded that HTRA2 is a SMAC-like inhibitor of IAP (inhibitor of apoptosis proteins) activity with a serine protease-dependent cell death-inducing activity.

Product Categories/Family for anti-HtrA3 antibody

Primary Antibodies; Signal Transduction

References

Nie, G.Y., et al., Biochem. J. 371 (Pt 1), 39-48 (2003)

NCBI and Uniprot Product Information

• NCBI GI #: 22129776
• NCBI GeneID: 94031
• NCBI Accession #: NP_444272.1
• NCBI GenBank Nucleotide #: NM_053044.4
• UniProt Accession #: P83110; D3ZA76
• Molecular Weight: 37,940 Da
• NCBI Official Full Name: serine protease HTRA3 isoform 1
• NCBI Official Synonym Full Names: HtrA serine peptidase 3
• NCBI Official Symbol: HTRA3
• NCBI Official Synonym Symbols: Prsp; Tasp
• NCBI Protein Information: serine protease HTRA3; probable serine protease HTRA3; pregnancy-related serine protease; high-temperature requirement factor A3
• UniProt Protein Name: Serine protease HTRA3
• Protein Family: Serine protease
• UniProt Gene Name: HTRA3
• UniProt Synonym Gene Names: PRSP
• UniProt Entry Name: HTRA3_HUMAN

Uniprot Description

HTRA3: Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly indirectly by degradation of these ECM proteoglycans. May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization. Belongs to the peptidase S1B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.-; Secreted, signal peptide; Protease; Secreted

Chromosomal Location of Human Ortholog: 4p16.1

Cellular Component: extracellular region

Molecular Function: insulin-like growth factor binding; protein binding; serine-type peptidase activity; serine-type endopeptidase activity; endopeptidase activity

Biological Process: regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; proteolysis; negative regulation of BMP signaling pathway