Mouse R-Spondin 2 ELISA Kit | RSPO2 elisa kit

Mouse R-Spondin 2 (RSPO2) ELISA Kit

Cat. Num. AAA2031892

• Gene Names: RSPO2; CRISTIN2
• Reactivity: Mouse
• Synonyms: R-Spondin 2; Mouse R-Spondin 2 (RSPO2) ELISA Kit; Roof plate-specific spondin-2; RSPO2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of R-Spondin 2 (RSPO2).; No significant cross-reactivity or interference between R-Spondin 2 (RSPO2) and analogues was observed.
• Sequence Length: 179

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 78-5000pg/mL
• Sensitivity: < 29pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level R-Spondin 2 (RSPO2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level R-Spondin 2 (RSPO2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve

Related Product Information for RSPO2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to R-Spondin 2 (RSPO2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to R-Spondin 2 (RSPO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain R-Spondin 2 (RSPO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of R-Spondin 2 (RSPO2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for RSPO2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 544583460
• NCBI GeneID: 340419
• NCBI Accession #: NP_001269792.1
• NCBI GenBank Nucleotide #: NM_001282863.1
• Molecular Weight: 20,972 Da
• NCBI Official Full Name: R-spondin-2 isoform 2
• NCBI Official Synonym Full Names: R-spondin 2
• NCBI Official Symbol: RSPO2
• NCBI Official Synonym Symbols: CRISTIN2
• NCBI Protein Information: R-spondin-2
• UniProt Protein Name: R-spondin-2
• Protein Family: R-spondin
• UniProt Gene Name: RSPO2
• UniProt Synonym Gene Names: hRspo2
• UniProt Entry Name: RSPO2_HUMAN

NCBI Description

This gene encodes a member of the R-spondin family of proteins. These proteins are secreted ligands of leucine-rich repeat containing G protein-coupled receptors that enhance Wnt signaling through the inhibition of ubiquitin E3 ligases. A chromosomal translocation including this locus that results in the formation of a gene fusion has been identified in multiple human cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Uniprot Description

R-spondin-2: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8q23.1

Cellular Component: cell surface; extracellular region

Molecular Function: heparin binding; protein binding; receptor binding

Biological Process: bone mineralization; embryonic forelimb morphogenesis; embryonic hindlimb morphogenesis; negative regulation of odontogenesis of dentine-containing teeth; osteoblast differentiation; Wnt receptor signaling pathway