Rat Beta-arrestin-1 ELISA Kit | Arrb1 elisa kit

Rat Beta-arrestin-1 ELISA Kit

Cat. Num. AAA2880669

• Gene Names: Arrb1; BARRES
• Reactivity: Rat
• Synonyms: Beta-arrestin-1; Rat Beta-arrestin-1 ELISA Kit; Arrestin beta-1; Arrb1; Arrb1 elisa kit

• Reactivity: Rat
• Specificity: Recombinant and natural Rat Beta-arrestin-1
• Sequence Length: 418

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.156-10 ng/mL
• Sensitivity: < 0.08 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=4.4%
• Inter-assay Precision: Inter-Assay CV: <=7.8%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Arrb1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6978537
• NCBI GeneID: 25387
• NCBI Accession #: NP_037042.1
• NCBI GenBank Nucleotide #: NM_012910.2
• UniProt Accession #: P29066
• Molecular Weight: 47,020 Da
• NCBI Official Full Name: beta-arrestin-1
• NCBI Official Synonym Full Names: arrestin, beta 1
• NCBI Official Symbol: Arrb1
• NCBI Official Synonym Symbols: BARRES
• NCBI Protein Information: beta-arrestin-1
• UniProt Protein Name: Beta-arrestin-1
• Protein Family: Beta-arrestin
• UniProt Gene Name: Arrb1
• UniProt Entry Name: ARRB1_RAT

Uniprot Description

ARRB1: regulates G-protein coupled receptors (GPCR) signaling by mediating both receptor desensitization and resensitization processes. Binds to GRK-phosphorylated receptor and sterically preclude its coupling to the cognate G- protein; the binding appears to require receptor determinants exposed only in the active receptor conformation. Targets many receptors for internalization by acting as endocytic adapters (CLASPs, clathrin-associated sorting proteins). Internalized arrestin-receptor complexes traffic to intracellular endosomes, where they remain uncoupled from G-proteins. Two different modes of arrestin-mediated internalization occur. Beta-arrestins function as multivalent adapter proteins that can switch the GPCR from a G-protein signaling mode that transmits short-lived signals from the plasma membrane via small molecule second messengers and ion channels to a beta-arrestin signaling mode that transmits a distinct set of signals that are initiated as the receptor internalizes and transits the intracellular compartment. Also involved in regulation of receptors other than GPCRs. Involved in Toll-like receptor and IL-1 receptor signaling through the interaction with TRAF6 which prevents TRAF6 autoubiquitination and oligomerization required for activation of NF-kappa-B and JUN. Binds phosphoinositides. Binds inositolhexakisphosphate (InsP6). Involved in IL8-mediated granule release in neutrophils. Interacts with phosphorylated ADRB2 and CHRM2. Interacts with SRC (via the SH3 domain and the protein kinase domain); the interaction is independent of the phosphorylation state of SRC C-terminus. Interacts with RAF1, CHUK, IKBKB and Nik. Interacts with DVL1 and DVL2; the interaction is enhanced by DVL phosphorylation. Interacts with IGF1R. Belongs to the arrestin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Cellular Component: basolateral plasma membrane; chromatin; coated pit; cytoplasm; cytoplasmic membrane-bound vesicle; cytoplasmic vesicle; cytosol; dendritic spine; intracellular; nucleoplasm; nucleus; plasma membrane; postsynaptic density; postsynaptic membrane; pseudopodium

Molecular Function: alpha-1A adrenergic receptor binding; alpha-1B adrenergic receptor binding; angiotensin receptor binding; caspase inhibitor activity; clathrin binding; estrogen receptor binding; follicle stimulating hormone receptor binding; G-protein-coupled receptor binding; GTPase activator activity; histone acetyltransferase activity; insulin-like growth factor receptor binding; mitogen-activated protein kinase kinase binding; phosphoprotein binding; protein binding; protein phosphorylated amino acid binding; transcription factor binding; ubiquitin protein ligase binding; V2 vasopressin receptor binding

Biological Process: activation of MAPK activity; arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; endocytosis; follicle-stimulating hormone signaling pathway; G-protein coupled receptor internalization; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; inhibition of NF-kappaB transcription factor; negative regulation of caspase activity; negative regulation of interleukin-6 production; negative regulation of interleukin-8 production; negative regulation of protein amino acid phosphorylation; negative regulation of protein ubiquitination; phototransduction; positive regulation of caspase activity; positive regulation of GTPase activity; positive regulation of histone acetylation; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein binding; positive regulation of protein ubiquitination; positive regulation of receptor internalization; positive regulation of Rho protein signal transduction; positive regulation of transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; protein transport; protein ubiquitination; regulation of cAMP biosynthetic process; regulation of cAMP catabolic process; regulation of G-protein coupled receptor protein signaling pathway; sensory perception of pain; sensory perception of touch; stress fiber formation; transcription from RNA polymerase II promoter