Typical Testing Data/Standard Curve (for reference only)
Mouse DNA topoisomerase 2-beta ELISA Kit | Top2b elisa kit
Mouse DNA topoisomerase 2-beta ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
TOP2B: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly involved in vitamin D- coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene. Belongs to the type II topoisomerase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell development/differentiation; Motility/polarity/chemotaxis; DNA-binding; Nucleolus; EC 5.99.1.3; Topoisomerase
Cellular Component: chromosome; cytoplasm; cytosol; heterochromatin; nucleoplasm; nucleus
Molecular Function: ATP binding; chromatin binding; DNA bending activity; DNA binding; DNA topoisomerase (ATP-hydrolyzing) activity; DNA topoisomerase activity; enzyme binding; histone deacetylase binding; isomerase activity; metal ion binding; nucleotide binding; protein binding; protein C-terminus binding; protein heterodimerization activity; protein kinase C binding; ribonucleoprotein binding
Biological Process: axonogenesis; DNA metabolic process; DNA topological change; forebrain development; neuron migration; resolution of meiotic joint molecules as recombinants; sister chromatid segregation
Research Articles on Top2b
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Product Notes
The Mouse Top2b top2b (Catalog #AAA2886193) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2886193 ELISA Kit recognizes Mouse Top2b. It is sometimes possible for the material contained within the vial of "DNA topoisomerase 2-beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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