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Typical Testing Data/Standard Curve (for reference only)

Rat beta Arrestin-1 (Beta Arrestin- 1) ELISA Kit | Arrb1 elisa kit

Rat beta Arrestin-1 (Beta Arrestin- 1) ELISA Kit

Gene Names
Arrb1; BARRES
Reactivity
Rat
Synonyms
beta Arrestin-1 (Beta Arrestin- 1); Rat beta Arrestin-1 (Beta Arrestin- 1) ELISA Kit; Beta-arrestin-1; beta Arrestin-1; Arrestin beta-1; Arrb1; Arrb1 elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of beta Arrestin-1. No significant cross-reactivity or interference between beta Arrestin-1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
15.625-1000pg/ml
Sensitivity
9.375pg/ml
Intra-assay Precision
CV<8%
Inter-assay Precision
CV<10%
Preparation and Storage
Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Arrb1 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,020 Da
NCBI Official Full Name
beta-arrestin-1
NCBI Official Synonym Full Names
arrestin, beta 1
NCBI Official Symbol
Arrb1
NCBI Official Synonym Symbols
BARRES
NCBI Protein Information
beta-arrestin-1
UniProt Protein Name
Beta-arrestin-1
Protein Family
UniProt Gene Name
Arrb1
UniProt Entry Name
ARRB1_RAT

Uniprot Description

ARRB1: regulates G-protein coupled receptors (GPCR) signaling by mediating both receptor desensitization and resensitization processes. Binds to GRK-phosphorylated receptor and sterically preclude its coupling to the cognate G- protein; the binding appears to require receptor determinants exposed only in the active receptor conformation. Targets many receptors for internalization by acting as endocytic adapters (CLASPs, clathrin-associated sorting proteins). Internalized arrestin-receptor complexes traffic to intracellular endosomes, where they remain uncoupled from G-proteins. Two different modes of arrestin-mediated internalization occur. Beta-arrestins function as multivalent adapter proteins that can switch the GPCR from a G-protein signaling mode that transmits short-lived signals from the plasma membrane via small molecule second messengers and ion channels to a beta-arrestin signaling mode that transmits a distinct set of signals that are initiated as the receptor internalizes and transits the intracellular compartment. Also involved in regulation of receptors other than GPCRs. Involved in Toll-like receptor and IL-1 receptor signaling through the interaction with TRAF6 which prevents TRAF6 autoubiquitination and oligomerization required for activation of NF-kappa-B and JUN. Binds phosphoinositides. Binds inositolhexakisphosphate (InsP6). Involved in IL8-mediated granule release in neutrophils. Interacts with phosphorylated ADRB2 and CHRM2. Interacts with SRC (via the SH3 domain and the protein kinase domain); the interaction is independent of the phosphorylation state of SRC C-terminus. Interacts with RAF1, CHUK, IKBKB and Nik. Interacts with DVL1 and DVL2; the interaction is enhanced by DVL phosphorylation. Interacts with IGF1R. Belongs to the arrestin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Cellular Component: basolateral plasma membrane; cytoplasmic membrane-bound vesicle; postsynaptic density; dendritic spine; coated pit; pseudopodium; cytosol; nucleoplasm; postsynaptic membrane; cytoplasm; plasma membrane; intracellular; cytoplasmic vesicle; chromatin; nucleus

Molecular Function: angiotensin receptor binding; protein phosphorylated amino acid binding; clathrin binding; follicle stimulating hormone receptor binding; mitogen-activated protein kinase kinase binding; caspase inhibitor activity; phosphoprotein binding; alpha-1B adrenergic receptor binding; transcription factor binding; GTPase activator activity; alpha-1A adrenergic receptor binding; V2 vasopressin receptor binding; insulin-like growth factor receptor binding; protein binding; histone acetyltransferase activity; G-protein-coupled receptor binding; ubiquitin protein ligase binding; estrogen receptor binding

Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; follicle-stimulating hormone signaling pathway; positive regulation of protein binding; activation of MAPK activity; positive regulation of receptor internalization; positive regulation of caspase activity; protein ubiquitination; negative regulation of caspase activity; sensory perception of pain; protein transport; negative regulation of protein amino acid phosphorylation; sensory perception of touch; phototransduction; negative regulation of interleukin-8 production; negative regulation of interleukin-6 production; regulation of cAMP biosynthetic process; arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; receptor-mediated endocytosis; positive regulation of Rho protein signal transduction; regulation of cAMP catabolic process; endocytosis; positive regulation of peptidyl-serine phosphorylation; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; G-protein coupled receptor internalization; positive regulation of protein ubiquitination; inhibition of NF-kappaB transcription factor; stress fiber formation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; negative regulation of protein ubiquitination; positive regulation of protein amino acid phosphorylation; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity

Research Articles on Arrb1

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Product Notes

The Rat Arrb1 arrb1 (Catalog #AAA7612433) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7612433 ELISA Kit recognizes Rat Arrb1. It is sometimes possible for the material contained within the vial of "beta Arrestin-1 (Beta Arrestin- 1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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