Rat beta Arrestin-1 (Beta Arrestin- 1) ELISA Kit | Arrb1 elisa kit

Rat beta Arrestin-1 (Beta Arrestin- 1) ELISA Kit

Cat. Num. AAA7612433

• Gene Names: Arrb1; BARRES
• Reactivity: Rat
• Synonyms: beta Arrestin-1 (Beta Arrestin- 1); Rat beta Arrestin-1 (Beta Arrestin- 1) ELISA Kit; Beta-arrestin-1; beta Arrestin-1; Arrestin beta-1; Arrb1; Arrb1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of beta Arrestin-1. No significant cross-reactivity or interference between beta Arrestin-1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Arrb1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 6978537
• NCBI GeneID: 25387
• NCBI Accession #: NP_037042.1
• NCBI GenBank Nucleotide #: NM_012910.2
• UniProt Accession #: P29066
• Molecular Weight: 47,020 Da
• NCBI Official Full Name: beta-arrestin-1
• NCBI Official Synonym Full Names: arrestin, beta 1
• NCBI Official Symbol: Arrb1
• NCBI Official Synonym Symbols: BARRES
• NCBI Protein Information: beta-arrestin-1
• UniProt Protein Name: Beta-arrestin-1
• Protein Family: Beta-arrestin
• UniProt Gene Name: Arrb1
• UniProt Entry Name: ARRB1_RAT

Uniprot Description

ARRB1: regulates G-protein coupled receptors (GPCR) signaling by mediating both receptor desensitization and resensitization processes. Binds to GRK-phosphorylated receptor and sterically preclude its coupling to the cognate G- protein; the binding appears to require receptor determinants exposed only in the active receptor conformation. Targets many receptors for internalization by acting as endocytic adapters (CLASPs, clathrin-associated sorting proteins). Internalized arrestin-receptor complexes traffic to intracellular endosomes, where they remain uncoupled from G-proteins. Two different modes of arrestin-mediated internalization occur. Beta-arrestins function as multivalent adapter proteins that can switch the GPCR from a G-protein signaling mode that transmits short-lived signals from the plasma membrane via small molecule second messengers and ion channels to a beta-arrestin signaling mode that transmits a distinct set of signals that are initiated as the receptor internalizes and transits the intracellular compartment. Also involved in regulation of receptors other than GPCRs. Involved in Toll-like receptor and IL-1 receptor signaling through the interaction with TRAF6 which prevents TRAF6 autoubiquitination and oligomerization required for activation of NF-kappa-B and JUN. Binds phosphoinositides. Binds inositolhexakisphosphate (InsP6). Involved in IL8-mediated granule release in neutrophils. Interacts with phosphorylated ADRB2 and CHRM2. Interacts with SRC (via the SH3 domain and the protein kinase domain); the interaction is independent of the phosphorylation state of SRC C-terminus. Interacts with RAF1, CHUK, IKBKB and Nik. Interacts with DVL1 and DVL2; the interaction is enhanced by DVL phosphorylation. Interacts with IGF1R. Belongs to the arrestin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Cellular Component: basolateral plasma membrane; cytoplasmic membrane-bound vesicle; postsynaptic density; dendritic spine; coated pit; pseudopodium; cytosol; nucleoplasm; postsynaptic membrane; cytoplasm; plasma membrane; intracellular; cytoplasmic vesicle; chromatin; nucleus

Molecular Function: angiotensin receptor binding; protein phosphorylated amino acid binding; clathrin binding; follicle stimulating hormone receptor binding; mitogen-activated protein kinase kinase binding; caspase inhibitor activity; phosphoprotein binding; alpha-1B adrenergic receptor binding; transcription factor binding; GTPase activator activity; alpha-1A adrenergic receptor binding; V2 vasopressin receptor binding; insulin-like growth factor receptor binding; protein binding; histone acetyltransferase activity; G-protein-coupled receptor binding; ubiquitin protein ligase binding; estrogen receptor binding

Biological Process: transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; follicle-stimulating hormone signaling pathway; positive regulation of protein binding; activation of MAPK activity; positive regulation of receptor internalization; positive regulation of caspase activity; protein ubiquitination; negative regulation of caspase activity; sensory perception of pain; protein transport; negative regulation of protein amino acid phosphorylation; sensory perception of touch; phototransduction; negative regulation of interleukin-8 production; negative regulation of interleukin-6 production; regulation of cAMP biosynthetic process; arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; receptor-mediated endocytosis; positive regulation of Rho protein signal transduction; regulation of cAMP catabolic process; endocytosis; positive regulation of peptidyl-serine phosphorylation; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; G-protein coupled receptor internalization; positive regulation of protein ubiquitination; inhibition of NF-kappaB transcription factor; stress fiber formation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; negative regulation of protein ubiquitination; positive regulation of protein amino acid phosphorylation; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity