Fractalkine (CX3CL1) Recombinant Protein | CX3CL1 recombinant protein

Fractalkine (CX3CL1)

Cat. Num. AAA517008

• Gene Names: CX3CL1; NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
• Applications: Cell Assay
• Synonyms: Fractalkine (CX3CL1); C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Neurotactin; Small-inducible cytokine D1.; CX3CL1 recombinant protein

• Host: E Coli
• Form/Format: Lyophilized from a 0.2 um filtered solution in PBS with BSA as a carrier protein

• Applicable Applications for CX3CL1 recombinant protein: Cell Assay
• Species: Human
• Endotoxin Level: <1.0EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: Reconstitute at 25ug/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Activity

(Sample Cell Assay Data: Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CX3CR1. The ED(50) for this effect is typically 20-100 ng/mL)

SDS-Page

(Purity: Recombinant Human Fractalkine(CX3CL1) resolved on a 17% SDS_PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250.)

Related Product Information for CX3CL1 recombinant protein

Recombinant Human Fractalkine (CX3CL1) was expressed in E Coli cells. The protein accession number is P78423
Fractalkine is a type I membrane protein that signals through the CX3CR1 receptor. The extracellular domain of Fractalkine is released by protease cleavage -producing soluble Fractalkine. Fractalkine has been found in many tissues and shown to be up-regulated in endothelial cells and microglia in response to inflammatory signals. Fractalkine is also involved in leukocyte adhesion as well as attracting monocytes, microglia cells and NK cells.

Product Categories/Family for CX3CL1 recombinant protein

Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4506857
• NCBI GeneID: 6376
• NCBI Accession #: NP_002987.1
• NCBI GenBank Nucleotide #: NP_002987.1
• UniProt Accession #: P78423
• NCBI Official Full Name: fractalkine isoform 1
• NCBI Official Synonym Full Names: C-X3-C motif chemokine ligand 1
• NCBI Official Symbol: CX3CL1
• NCBI Official Synonym Symbols: NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
• NCBI Protein Information: fractalkine
• UniProt Protein Name: Fractalkine
• Protein Family: Fractalkine
• UniProt Gene Name: CX3CL1
• UniProt Synonym Gene Names: FKN; NTT; SCYD1
• UniProt Entry Name: X3CL1_HUMAN

NCBI Description

This gene belongs to the CX3C subgroup of chemokines, characterized by the number of amino acids located between the conserved cysteine residues. This is the only member of the CX3C subgroup, which contains three amino acids between cysteine residues, resulting in a Cys-X-X-X-Cys configuration. The encoded protein contains an extended mucin-like stalk with a chemokine domain on top, and exists in both a membrane-anchored form where it acts as a binding molecule, or, in soluble form, as a chemotactic cytokine. The mature form of this protein can be cleaved at the cell surface, yielding different soluble forms that can interact with the G-protein coupled receptor, C-X3-C motif chemokine receptor 1 gene product. This gene plays a role in a wide range of diseases, including cancer, vasculitis, neuropathies, atherosclerosis, inflammatory diseases, and in human immunodeficiency virus infections. [provided by RefSeq, Sep 2017]

Uniprot Description

CX3CL1: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Belongs to the intercrine delta family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: extracellular space; cell surface; integral to membrane; extracellular region; plasma membrane

Molecular Function: protein binding; chemokine activity; receptor binding

Biological Process: neutrophil chemotaxis; positive regulation of transforming growth factor-beta1 production; leukocyte chemotaxis; cytokine and chemokine mediated signaling pathway; defense response; chemotaxis; angiogenesis involved in wound healing; leukocyte adhesive activation; macrophage chemotaxis; positive regulation of angiogenesis; positive regulation of calcium-independent cell-cell adhesion; immune response; cell adhesion; lymphocyte chemotaxis; positive regulation of inflammatory response