EGF recombinant protein

EGF

Cat. Num. AAA516949

• Gene Names: EGF; URG; HOMG4
• Applications: Cell Assay
• Synonyms: EGF; Urogastrone; Recombinant Human Epidermal Growth Factor (EGF).; EGF recombinant protein

• Host: E Coli
• Form/Format: Lyophilized from a 0.2 um filtered solution in tris buffer with NaCl

• Applicable Applications for EGF recombinant protein: Cell Assay
• Species: Human
• Endotoxin Level: <1.0EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1mg/ml. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Activity

(Sample Cell Assay Data: The ED50 as determined by a cell proliferation assay using 3T3 cells was )

SDS-Page

(Purity: Recombinant Human EGF resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250.)

Related Product Information for EGF recombinant protein

Recombinant Human EGF was expressed in E Coli cells. The protein accession number is P01133
Epidermal growth factor (EGF) is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, inhibition of gastric acid secretion, oncogenesis and wound healing. EGF shares strong sequential and functional homology with the type-alpha transforming growth factor, which is a competitor for EGF receptor sites. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. The EGF receptor is expressed in almost all types of tissues with the exception of the parietal endoderm, mature skeletal muscles and hematopoietic tissue. Recombinant Human EGF is a globular protein that displays the EGF-like domain; characterized by three intra-molecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues. Mature human EGF shares 70% amino acid sequence identity with its counterparts from mouse and rat.

Product Categories/Family for EGF recombinant protein

Growth Factors; Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 251757262
• NCBI GeneID: 1950
• UniProt Accession #: P01133; Q52LZ6; B4DRK7; E7EVD2; E9PBF0
• Molecular Weight: 1207
• NCBI Official Full Name: Pro-epidermal growth factor
• NCBI Official Synonym Full Names: epidermal growth factor
• NCBI Official Symbol: EGF
• NCBI Official Synonym Symbols: URG; HOMG4
• NCBI Protein Information: pro-epidermal growth factor; beta-urogastrone
• UniProt Protein Name: Pro-epidermal growth factor
• Protein Family: EGF-like domain containing protein
• UniProt Gene Name: EGF
• UniProt Synonym Gene Names: EGF
• UniProt Entry Name: EGF_HUMAN