IL36G recombinant protein

IL36G

Cat. Num. AAA517126

• Gene Names: IL36G; IL1E; IL1F9; IL1H1; IL-1F9; IL-1H1; IL1RP2; IL-1RP2
• Applications: Cell Assay
• Synonyms: IL36G; IL1RP2; IL-1 epsilon; IL1F9; IL1H1; IL36G recombinant protein

• Host: E Coli
• Form/Format: Lyophilized from a 0.2 um filtered solution in Tris and NaCl

• Applicable Applications for IL36G recombinant protein: Cell Assay
• Species: Human
• Endotoxin Level: <1.0EU/ug of recombinant protein as determined by the LAL method
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1mg/ml. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Activity

(Sample Cell Assay Data: The ED (50) was determined by the dose-dependent proliferation of GieNo3B11 cells and was found to be )

SDS-Page

(Purity: Recombinant Human IL36G resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250)

Related Product Information for IL36G recombinant protein

Recombinant Human IL36G was expressed in E Coli cells. The protein accession number is Q9NZH8
Human IL36G is a secreted member of the IL1 family, one of three IL36 genes in humans. The IL1 family members display a conserved 12 b-strand and a b-trefoil configuration, likely due to ascendance from a mutual ancenstral gene. IL36G is expressed primarily in tissues containing epithelial cells, and is both intracellular and secreted. Its primary receptor appears to be a combination of IL-1rp2 and IL-1Racp, the first being primarily expressed on dendritic cells and the second being more widely expressed. Binding of IL36G ligands to this receptor is one of the factors which induces dendritic cell maturation. Its expression is also an agonist for pro-inflammatory cytokines through the NF-kB and Map kinase pathways. Recombinant human IL36G migrates as an 18.9kDa protein on SDS-PAGE and shares approximately 52% sequence identity with mouse IL36G.

Product Categories/Family for IL36G recombinant protein

Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 9665234
• NCBI GeneID: 56300
• NCBI Accession #: NP_062564.1
• NCBI GenBank Nucleotide #: NM_019618.3
• UniProt Accession #: Q9NZH8; Q56B91; Q6UVX7; Q7RTZ9
• Molecular Weight: 18,721 Da
• NCBI Official Full Name: interleukin-36 gamma isoform 1
• NCBI Official Synonym Full Names: interleukin 36, gamma
• NCBI Official Symbol: IL36G
• NCBI Official Synonym Symbols: IL1E; IL1F9; IL1H1; IL-1F9; IL-1H1; IL1RP2; IL-1RP2
• NCBI Protein Information: interleukin-36 gamma; IL-1 epsilon; IL-1-epsilon; IL-1(EPSILON); interleukin-1 epsilon; IL-1 related protein 2; IL-1-related protein 2; interleukin-1 homolog 1; interleukin-1 family member 9; interleukin 1 family, member 9; interleukin 1-related protein 2
• UniProt Protein Name: Interleukin-36 gamma
• Protein Family: Interleukin
• UniProt Gene Name: IL36G
• UniProt Synonym Gene Names: IL1E; IL1F9; IL1H1; IL1RP2; IL-1RP2; IL-1 epsilon; IL-1F9; IL-1H1
• UniProt Entry Name: IL36G_HUMAN

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. The activity of this cytokine is mediated by interleukin 1 receptor-like 2 (IL1RL2/IL1R-rp2), and is specifically inhibited by interleukin 1 family, member 5 (IL1F5/IL-1 delta). Interferon-gamma, tumor necrosis factor-alpha and interleukin 1, beta (IL1B) are reported to stimulate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

Il1f9: Function as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. Could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1), that is present in epithelial barriers and takes part in local inflammatory response. Belongs to the IL-1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 2q12-q21

Cellular Component: extracellular space

Molecular Function: interleukin-1 receptor binding; cytokine activity

Biological Process: cell-cell signaling; cytokine and chemokine mediated signaling pathway; positive regulation of interleukin-6 production; innate immune response; negative regulation of myoblast differentiation; inflammatory response