Rat Aquaporin-9 ELISA Kit | Aqp9 elisa kit

Rat Aquaporin-9 ELISA Kit

Cat. Num. AAA288197

• Reactivity: Rat
• Synonyms: Aquaporin-9; Rat Aquaporin-9 ELISA Kit; Aqp9 elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural rat AQP-9. No significant cross-reactivity or interference was observed.
• Sequence Length: 295

• Assay Type: Sandwich
• Detection Range: 0.78-50 ng/ml
• Sensitivity: 0.421 ng/mL
• Intra-Assay CV: <=4.9%
• Inter-Assay CV: <=9.1%
• Recovery: 106%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Aqp9 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AQP-9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AQP-9 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AQP-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of AQP-9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 12621144
• NCBI GeneID: 65054
• NCBI Accession #: NP_075249.1
• NCBI GenBank Nucleotide #: NM_022960.2
• UniProt Accession #: P56627; O88815
• Molecular Weight: 31,871 Da
• NCBI Official Full Name: aquaporin-9
• NCBI Official Synonym Full Names: aquaporin 9
• NCBI Official Symbol: Aqp9
• NCBI Protein Information: aquaporin-9
• UniProt Protein Name: Aquaporin-9
• Protein Family: Aquaporin
• UniProt Gene Name: Aqp9
• UniProt Synonym Gene Names: AQP-9
• UniProt Entry Name: AQP9_RAT

NCBI Description

solute channel involved in passage of carbamides, polyols, purines, and pyrimidines [RGD, Feb 2006]

Uniprot Description

AQP9: Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na(+), K(+), Cl(-), and deprotonated monocarboxylates are excluded. Also permeable to urea and glycerol. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Channel, misc.; Transporter, aquaporin family; Transporter; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: basolateral plasma membrane; integral to membrane; intracellular membrane-bound organelle

Molecular Function: glycerol channel activity; purine transmembrane transporter activity; pyrimidine transmembrane transporter activity; urea transmembrane transporter activity; water channel activity

Biological Process: amine transport; canalicular bile acid transport; glycerol transport; polyol transport; purine transport; pyrimidine transport; response to mercury ion; response to organic substance; water transport