Mouse Beta-arrestin-2 ELISA Kit | Arrb2 elisa kit

Mouse Beta-arrestin-2 ELISA Kit

Cat. Num. AAA288411

• Gene Names: Arrb2; AI326910; AW122872
• Reactivity: Mouse
• Synonyms: Beta-arrestin-2; Mouse Beta-arrestin-2 ELISA Kit; Arrb2 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse Beta-arrestin-2. No significant cross-reactivity or interference was observed.
• Sequence Length: 406

• Assay Type: Sandwich
• Detection Range: 0.156-10 ng/ml
• Sensitivity: 0.081 ng/mL
• Intra-Assay CV: <=6.9%
• Inter-Assay CV: <=8.8%
• Recovery: 106%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Arrb2 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-arrestin-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Beta-arrestin-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Beta-arrestin-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Beta-arrestin-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 405778340
• NCBI GeneID: 216869
• NCBI Accession #: NP_001258287.1
• NCBI GenBank Nucleotide #: NM_001271358.1
• UniProt Accession #: Q91YI4; Q3TCM2; Q5F2D8; Q5F2E0
• Molecular Weight: 47,440 Da
• NCBI Official Full Name: beta-arrestin-2 isoform a
• NCBI Official Synonym Full Names: arrestin, beta 2
• NCBI Official Symbol: Arrb2
• NCBI Official Synonym Symbols: AI326910; AW122872
• NCBI Protein Information: beta-arrestin-2
• UniProt Protein Name: Beta-arrestin-2
• Protein Family: Beta-arrestin
• UniProt Gene Name: Arrb2
• UniProt Entry Name: ARRB2_MOUSE

Uniprot Description

ARRB2: a member of the arrestin/beta-arrestin protein family. These proteins participate in agonist-mediated desensitization of G-protein-coupled receptors. Acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Expressed at high levels in the CNS and peripheral blood leukocytes.

Protein type: Adaptor/scaffold

Cellular Component: basolateral plasma membrane; coated pit; cytoplasm; cytoplasmic vesicle; dendritic spine; endocytic vesicle; intracellular; membrane; nucleus; plasma membrane; postsynaptic density; postsynaptic membrane

Molecular Function: alpha-1A adrenergic receptor binding; alpha-1B adrenergic receptor binding; angiotensin receptor binding; D1 dopamine receptor binding; enzyme binding; follicle stimulating hormone receptor binding; G-protein-coupled receptor binding; low-density lipoprotein receptor binding; mitogen-activated protein kinase binding; platelet activating factor receptor binding; protein binding; protein complex binding; protein complex scaffold; protein domain specific binding; protein kinase B binding; receptor binding; type 1 angiotensin receptor binding; type 2A serotonin receptor binding; ubiquitin protein ligase binding

Biological Process: adult walking behavior; arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; detection of temperature stimulus involved in sensory perception of pain; endocytosis; follicle-stimulating hormone signaling pathway; G-protein coupled receptor internalization; G-protein coupled receptor protein signaling pathway; inhibition of NF-kappaB transcription factor; negative regulation of caspase activity; negative regulation of interleukin-1 beta production; negative regulation of interleukin-12 production; negative regulation of interleukin-6 production; negative regulation of natural killer cell mediated cytotoxicity; negative regulation of protein ubiquitination; negative regulation of signal transduction; negative regulation of toll-like receptor signaling pathway; negative regulation of tumor necrosis factor production; positive regulation of calcium ion transport; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of protein ubiquitination; positive regulation of receptor internalization; positive regulation of synaptic transmission, dopaminergic; proteasomal ubiquitin-dependent protein catabolic process; protein transport; protein ubiquitination; receptor internalization; regulation of G-protein coupled receptor protein signaling pathway; regulation of protein amino acid phosphorylation; signal transduction; transcription from RNA polymerase II promoter; transforming growth factor beta receptor signaling pathway; transport