Rat Aquaporin-3 ELISA Kit | Aqp3 elisa kit

Rat Aquaporin-3 ELISA Kit

Cat. Num. AAA288344

• Reactivity: Rat
• Synonyms: Aquaporin-3; Rat Aquaporin-3 ELISA Kit; Aqp3 elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural rat AQP-3. No significant cross-reactivity or interference was observed.
• Sequence Length: 292

• Assay Type: Sandwich
• Detection Range: 0.156-10 ng/ml
• Sensitivity: 0.078 ng/mL
• Intra-Assay CV: <=4.3%
• Inter-Assay CV: <=9.2%
• Recovery: 96%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Aqp3 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AQP-3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AQP-3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AQP-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of AQP-3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13928980
• NCBI GeneID: 65133
• NCBI Accession #: NP_113891.1
• NCBI GenBank Nucleotide #: NM_031703.1
• UniProt Accession #: P47862; A0JPL5
• Molecular Weight: 31,384 Da
• NCBI Official Full Name: aquaporin-3
• NCBI Official Synonym Full Names: aquaporin 3
• NCBI Official Symbol: Aqp3
• NCBI Protein Information: aquaporin-3
• UniProt Protein Name: Aquaporin-3
• Protein Family: Aquaporin
• UniProt Gene Name: Aqp3
• UniProt Synonym Gene Names: AQP-3
• UniProt Entry Name: AQP3_RAT

NCBI Description

acts as a water transport channel in the collecting duct of the renal medulla [RGD, Feb 2006]

Uniprot Description

Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism.