SIRT7 sirna

SIRT7 siRNA (Human)

Cat. Num. AAA8235527

• Gene Names: SIRT7; SIR2L7
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SIRT7; SIRT7 siRNA (Human); SIR2L7; NAD-dependent protein deacetylase sirtuin-7; Regulatory protein SIR2 homolog 7; SIR2-like protein 7; SIRT7 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SIRT7 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 400

• Applicable Applications for SIRT7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SIRT7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SIRT7 sirna

siRNA to inhibit SIRT7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7706712
• NCBI GeneID: 51547
• NCBI Accession #: NP_057622.1
• NCBI GenBank Nucleotide #: NM_016538.2
• UniProt Accession #: Q9NRC8; Q3MIK4; Q9NSZ6; Q9NUS6; A8K2K0; B3KSU8
• Molecular Weight: 35,851 Da
• NCBI Official Full Name: NAD-dependent protein deacetylase sirtuin-7
• NCBI Official Synonym Full Names: sirtuin 7
• NCBI Official Symbol: SIRT7
• NCBI Official Synonym Symbols: SIR2L7
• NCBI Protein Information: NAD-dependent protein deacetylase sirtuin-7
• UniProt Protein Name: NAD-dependent protein deacetylase sirtuin-7
• Protein Family: NAD-dependent protein deacetylase
• UniProt Gene Name: SIRT7
• UniProt Synonym Gene Names: SIR2L7
• UniProt Entry Name: SIR7_HUMAN

NCBI Description

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008]

Uniprot Description

SIRT7: NAD-dependent protein deacetylase that specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac). In contrast to other histone deacetylases, displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors. SIRT7 thereby acts as a transcription repressor. Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells. These data suggest that SIRT7 may play a key role in oncogenic transformation by suppresses expression of tumor suppressor genes by locus-specific deacetylation of H3K18Ac at promoter regions. Also required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis: promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. May also deacetylate p53/TP53 and promotes cell survival, however such data need additional confirmation. Belongs to the sirtuin family. Class IV subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Deacetylase; Nucleolus; EC 3.5.1.-

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: nucleolus organizer region; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; metal ion binding; chromatin binding

Biological Process: activation of transcription on exit from mitosis; negative regulation of transcription from RNA polymerase II promoter; rRNA transcription