Sheep ApoF ELISA Kit | ApoF elisa kit
Sheep ApoF (Apolipoprotein F) ELISA Kit
Gene Names
APOF; LTIP; Apo-F
Reactivity
Sheep
Synonyms
ApoF; Sheep ApoF (Apolipoprotein F) ELISA Kit; ApoF elisa kit
Reactivity
Sheep
Specificity
This kit recognizes natural and recombinant Sheep ApoF. No significant cross-reactivity or interference between Sheep ApoF and analogues was observed.
Sequence Length
326
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.781-50ng/mL
Sensitivity
Min: 0.469ng/mL; Max: 50ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ApoF elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep ApoF concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ApoF. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ApoF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ApoF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ApoF. You can calculate the concentration of ApoF in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ApoF. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ApoF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ApoF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ApoF. You can calculate the concentration of ApoF in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,399 Da
NCBI Official Full Name
apolipoprotein F
NCBI Official Synonym Full Names
apolipoprotein F
NCBI Official Symbol
APOF
NCBI Official Synonym Symbols
LTIP; Apo-F
NCBI Protein Information
apolipoprotein F; lipid transfer inhibitor protein
UniProt Protein Name
Apolipoprotein F
Protein Family
UniProt Gene Name
APOF
UniProt Synonym Gene Names
Apo-F; LTIP
UniProt Entry Name
APOF_HUMAN
NCBI Description
The product of this gene is one of the minor apolipoproteins found in plasma. This protein forms complexes with lipoproteins and may be involved in transport and/or esterification of cholesterol. [provided by RefSeq, Jul 2008]
Uniprot Description
APOF: Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 12q13.3
Cellular Component: extracellular region
Molecular Function: lipid transporter activity; cholesterol binding; receptor binding
Biological Process: cholesterol metabolic process; triacylglycerol metabolic process; cholesterol efflux; lipid metabolic process; lipid transport
