Rabbit anti-Mouse, Human Jak3 Polyclonal Antibody | anti-Jak3 antibody

Jak3, NT (Jak3, Tyrosine-protein kinase JAK3, Janus kinase 3) (MaxLight 750)

Cat. Num. AAA6311530

• Gene Names: Jak3; fae; wil
• Reactivity: Mouse, Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Jak3; Polyclonal Antibody; NT (Jak3; Tyrosine-protein kinase JAK3; Janus kinase 3) (MaxLight 750); Janus kinase 3; anti-Jak3 antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Jak3. Species Crossreactivity: human
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
• Sequence Length: 1100

• Applicable Applications for anti-Jak3 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 133-161 in the N-terminal region of mouse Jak3, UniProt Accession #Q62137.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS6011099. This demonstrates that MBS6011099 detected the Jak3 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse stomach tissue lysates (35ug/lane) usingMBS6011099. This demonstrates that MBS6011099 detected the Jak3 protein (arrow).)

Related Product Information for anti-Jak3 antibody

Tyrosine kinase of the non-receptor type, involved in the interleukin-2 and interleukin-4 signaling pathway. Phosphorylates STAT6, IRS1, IRS2 and PI3K.

Product Categories/Family for anti-Jak3 antibody

Antibodies; Protein Kinases

References

1. Martin, P., et al. Mol. Cell. Biol. 30(20):4877-4889(2010). 2. Lange, C., et al. J. Neurochem. 113(5):1210-1220(2010). 3. Shi, M., et al. J. Immunol. 183(7):4493-4501(2009). 4. Chang, B.Y., et al. J. Immunol. 183(3):2183-2192(2009). 5. Rivas-Caicedo, A., et al. PLoS ONE 4 (9), E7066 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 46559418
• NCBI GeneID: 16453
• NCBI Accession #: NP_034719.2; NP_001177759.1
• NCBI GenBank Nucleotide #: NP_034719.2
• UniProt Accession #: Q62137
• NCBI Official Full Name: tyrosine-protein kinase JAK3
• NCBI Official Synonym Full Names: Janus kinase 3
• NCBI Official Symbol: Jak3
• NCBI Official Synonym Symbols: fae; wil
• NCBI Protein Information: tyrosine-protein kinase JAK3
• UniProt Protein Name: Tyrosine-protein kinase JAK3
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Jak3
• UniProt Synonym Gene Names: JAK-3
• UniProt Entry Name: JAK3_MOUSE

Uniprot Description

JAK3: a non-receptor tyrosine-kinase of the Jak family expressed predominantly in immune cells. Mediates signal transduction via the common gamma-chain of cytokine receptors, including IL-2, -4, -7, -9, and -15. Interacts with members of the STAT (signal transduction and activators of transcription) family. Interacts with STAM2 and SHB. Contains two protein kinase domains. Mutations that abrogate JAK3 function cause an autosomal SCID (severe combined immunodeficiency disease). Inhibitor: R017s for organ transplants. Three differentially spliced isoforms have been described.

Protein type: Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; TK group; JakA family

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; membrane; cytoplasm; intracellular; cytosol

Molecular Function: transferase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; kinase activity; protein phosphatase binding; ATP binding; hormone receptor binding; protein kinase activity

Biological Process: positive regulation of immune response; peptidyl-tyrosine phosphorylation; negative regulation of T-helper 1 cell differentiation; negative regulation of FasL biosynthetic process; negative regulation of interleukin-10 production; protein amino acid autophosphorylation; positive regulation of activated T cell proliferation; protein amino acid phosphorylation; enzyme linked receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; erythrocyte differentiation; positive regulation of T cell proliferation; T cell homeostasis; inflammatory response; negative regulation of interleukin-12 production; cell migration; immune system process; cytokine and chemokine mediated signaling pathway; tyrosine phosphorylation of Stat5 protein; lymph node development; negative regulation of dendritic cell cytokine production; tyrosine phosphorylation of STAT protein; B cell differentiation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of calcium ion transport; negative regulation of T cell activation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway