Chicken Apolipoprotein F ELISA Kit | APOF elisa kit

Chicken Apolipoprotein F ELISA Kit

Cat. Num. AAA742015

• Gene Names: APOF; LTIP; Apo-F
• Reactivity: Chicken
• Synonyms: Apolipoprotein F; Chicken Apolipoprotein F ELISA Kit; APOF elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This APOF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken APOF. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This APOF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken APOF. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOF elisa kit

Principle of the Assay: APOF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-APOF antibody and an APOF-HRP conjugate. The assay sample and buffer are incubated together with APOF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APOF concentration since APOF from samples and APOF-HRP conjugate compete for the anti-APOF antibody binding site. Since the number of sites is limited, as more sites are occupied by APOF from the sample, fewer sites are left to bind APOF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APOF concentration in each sample is interpolated from this standard curve.

Product Categories/Family for APOF elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 4502165
• NCBI GeneID: 319
• NCBI Accession #: NP_001629.1
• NCBI GenBank Nucleotide #: NM_001638.2
• Molecular Weight: 35,399 Da
• NCBI Official Full Name: apolipoprotein F
• NCBI Official Synonym Full Names: apolipoprotein F
• NCBI Official Symbol: APOF
• NCBI Official Synonym Symbols: LTIP; Apo-F
• NCBI Protein Information: apolipoprotein F; lipid transfer inhibitor protein
• UniProt Protein Name: Apolipoprotein F
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOF
• UniProt Synonym Gene Names: Apo-F; LTIP
• UniProt Entry Name: APOF_HUMAN

NCBI Description

The product of this gene is one of the minor apolipoproteins found in plasma. This protein forms complexes with lipoproteins and may be involved in transport and/or esterification of cholesterol. [provided by RefSeq, Jul 2008]

Uniprot Description

APOF: Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: extracellular region

Molecular Function: lipid transporter activity; cholesterol binding; receptor binding

Biological Process: cholesterol metabolic process; triacylglycerol metabolic process; cholesterol efflux; lipid metabolic process; lipid transport