Mouse Apolipoprotein C3 ELISA Kit | APOC3 elisa kit

Mouse Apolipoprotein C3 ELISA Kit

Cat. Num. AAA7937

• Gene Names: APOC3; HALP2; APOCIII
• Reactivity: Mouse
• Synonyms: Apolipoprotein C3; Mouse Apolipoprotein C3 ELISA Kit; APOC3 elisa kit

• Reactivity: Mouse
• Specificity: No significant cross-reactivity or interference between Mouse APOC3 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 2.5 mg/dl - 80 mg/dl.
• Sensitivity: 0.5 mg/dl.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated APOC3 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse APOC3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add APOC3 and APOC3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of APOC3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for APOC3 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated APOC3 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse APOC3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add APOC3 and APOC3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of APOC3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557323
• NCBI GeneID: 345
• NCBI Accession #: NP_000031.1
• NCBI GenBank Nucleotide #: NM_000040.1
• UniProt Accession #: P02656; Q08E83; Q6Q786
• Molecular Weight: 10,852 Da
• NCBI Official Full Name: apolipoprotein C-III
• NCBI Official Synonym Full Names: apolipoprotein C-III
• NCBI Official Symbol: APOC3
• NCBI Official Synonym Symbols: HALP2; APOCIII
• NCBI Protein Information: apolipoprotein C-III; apo-CIII; apoC-III; apolipoprotein C3
• UniProt Protein Name: Apolipoprotein C-III
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOC3
• UniProt Synonym Gene Names: Apo-CIII; ApoC-III
• UniProt Entry Name: APOC3_HUMAN

NCBI Description

Apolipoprotein C-III is a very low density lipoprotein (VLDL) protein. APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to delay catabolism of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia. [provided by RefSeq, Jul 2008]

Uniprot Description

APOC3: Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. Defects in APOC3 are the cause of hyperalphalipoproteinemia type 2 (HALP2). HALP2 is a condition characterized by high levels of high density lipoprotein (HDL) and increased HDL cholesterol levels. Belongs to the apolipoprotein C3 family.

Protein type: Secreted; Secreted, signal peptide; Lipid-binding

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: extracellular space; chylomicron; early endosome; extracellular region

Molecular Function: lipase inhibitor activity; cholesterol binding; phospholipid binding; enzyme regulator activity

Biological Process: response to drug; cholesterol metabolic process; response to peptide hormone stimulus; phototransduction, visible light; regulation of Cdc42 protein signal transduction; lipoprotein metabolic process; cholesterol efflux; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; triacylglycerol metabolic process; reverse cholesterol transport; negative regulation of lipid catabolic process; phospholipid efflux; lipoprotein transport; triacylglycerol catabolic process; negative regulation of lipoprotein lipase activity; negative regulation of receptor-mediated endocytosis; negative regulation of lipid metabolic process; retinoid metabolic process; inflammatory response; triacylglycerol mobilization; negative regulation of fatty acid biosynthetic process; response to nutrient

Disease: Apolipoprotein C-iii Deficiency