Western Blot (WB)
(Western Blot; Sample: Recombinant C4a, Rat.)
Rabbit anti-Rat Complement Component 4a (C4a) Polyclonal Antibody | anti-C4a antibody
Polyclonal Antibody to Complement Component 4a (C4a)
Gene Names
C4a; C4; C4b; C4-1
Reactivity
Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Complement Component 4a (C4a); Polyclonal Antibody; Polyclonal Antibody to Complement Component 4a (C4a); C4a anaphylatoxin; anti-C4a antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against C4a. It has been selected for its ability to recognize C4a in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
0.24mg/ml (varies by lot)
Applicable Applications for anti-C4a antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.5-2ug/mL; 1:120-480
IHC: 5-20ug/mL; 1:12-48
ICC: 5-20ug/mL; 1:12-48
IHC: 5-20ug/mL; 1:12-48
ICC: 5-20ug/mL; 1:12-48
Cross Reactivity
Rat
Conjugation
None
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot; Sample: Recombinant C4a, Rat.)
Western Blot (WB)
(Western Blot; Sample: Recombinant C4a, Rat.)
Product Categories/Family for anti-C4a antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
192,163 Da
NCBI Official Full Name
complement C4
NCBI Official Synonym Full Names
complement component 4A (Rodgers blood group)
NCBI Official Symbol
C4a
NCBI Official Synonym Symbols
C4; C4b; C4-1
NCBI Protein Information
complement C4
UniProt Protein Name
Complement C4
Protein Family
UniProt Gene Name
C4
UniProt Synonym Gene Names
C4a
UniProt Entry Name
CO4_RAT
NCBI Description
complement protein, part of the classical activation pathway; localizes to the class III region of the MHC [RGD, Feb 2006]
Uniprot Description
C4A: Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory processes. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes
Protein type: Secreted; Secreted, signal peptide; Inhibitor
Cellular Component: extracellular region; extracellular space
Molecular Function: complement component C1q binding; endopeptidase inhibitor activity
Biological Process: complement activation; complement activation, classical pathway; inflammatory response; innate immune response
Research Articles on C4a
Similar Products
Product Notes
The C4a c4 (Catalog #AAA2111868) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Complement Component 4a (C4a) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Complement Component 4a (C4a) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.5-2ug/mL; 1:120-480 IHC: 5-20ug/mL; 1:12-48 ICC: 5-20ug/mL; 1:12-48. Researchers should empirically determine the suitability of the C4a c4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Complement Component 4a (C4a), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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