Human Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2) RTU ELISA Kit | NR1D2 rtu elisa kit

Human Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2) ELISA Kit

Cat. Num. AAA4503585

• Gene Names: NR1D2; RVR; BD73; EAR-1R; REVERBB; REVERBbeta
• Reactivity: Human
• Synonyms: Nuclear Receptor Subfamily 1; Group D; Member 2 (NR1D2); Human Nuclear Receptor Subfamily 1; Member 2 (NR1D2) ELISA Kit; HZF2; BD73; EAR-1r; RVR; Rev-ErbA Beta; Orphan nuclear hormone receptor BD73; Rev-erb alpha-related receptor; V-erbA-related protein 1-related; NR1D2 rtu elisa kit

• Reactivity: Human
• Sequence Length: 579

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.128ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NR1D2 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NR1D2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NR1D2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NR1D2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NR1D2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 224177491
• NCBI GeneID: 9975
• NCBI Accession #: NP_005117.3
• NCBI GenBank Nucleotide #: NM_005126.4
• UniProt Accession #: Q14995; O00402; Q86XD4; B2R8Q3
• Molecular Weight: 64,625 Da
• NCBI Official Full Name: nuclear receptor subfamily 1 group D member 2 isoform 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1 group D member 2
• NCBI Official Symbol: NR1D2
• NCBI Official Synonym Symbols: RVR; BD73; EAR-1R; REVERBB; REVERBbeta
• NCBI Protein Information: nuclear receptor subfamily 1 group D member 2
• UniProt Protein Name: Nuclear receptor subfamily 1 group D member 2
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR1D2
• UniProt Synonym Gene Names: RVR; EAR-1R

NCBI Description

This gene encodes a member of the nuclear hormone receptor family, specifically the NR1 subfamily of receptors. The encoded protein functions as a transcriptional repressor and may play a role in circadian rhythms and carbohydrate and lipid metabolism. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2009]

Uniprot Description

Transcriptional repressor which coordinates circadian rhythm and metabolic pathways in a heme-dependent manner. Integral component of the complex transcription machinery that governs circadian rhythmicity and forms a critical negative limb of the circadian clock by directly repressing the expression of core clock components ARNTL/BMAL1 and CLOCK. Also regulates genes involved in metabolic functions, including lipid metabolism and the inflammatory response. Acts as a receptor for heme which stimulates its interaction with the NCOR1/HDAC3 corepressor complex, enhancing transcriptional repression. Recognizes two classes of DNA response elements within the promoter of its target genes and can bind to DNA as either monomers or homodimers, depending on the nature of the response element. Binds as a monomer to a response element composed of the consensus half-site motif 5'-[A/G]GGTCA-3' preceded by an A/T-rich 5' sequence (RevRE), or as a homodimer to a direct repeat of the core motif spaced by two nuclegotides (RevDR-2). Acts as a potent competitive repressor of ROR alpha (RORA) function and also negatively regulates the expression of NR1D1. Regulates lipid and energy homeostasis in the skeletal muscle via repression of genes involved in lipid metabolism and myogenesis including: CD36, FABP3, FABP4, UCP3, SCD1 and MSTN. Regulates hepatic lipid metabolism via the repression of APOC3. Represses gene expression at a distance in macrophages by inhibiting the transcription of enhancer-derived RNAs (eRNAs). In addition to its activity as a repressor, can also act as a transcriptional activator. Acts as a transcriptional activator of the sterol regulatory element-binding protein 1 (SREBF1) and the inflammatory mediator interleukin-6 (IL6) in the skeletal muscle.