Typical Testing Data/Standard Curve (for reference only)
Monkey Apolipoprotein C2 ELISA Kit | APOC2 elisa kit
Monkey Apolipoprotein C2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: APOC2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-APOC2 antibody and an APOC2-HRP conjugate. The assay sample and buffer are incubated together with APOC2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APOC2 concentration since APOC2 from samples and APOC2-HRP conjugate compete for the anti-APOC2 antibody binding site. Since the number of sites is limited, as more sites are occupied by APOC2 from the sample, fewer sites are left to bind APOC2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APOC2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a lipid-binding protein belonging to the apolipoprotein gene family. The protein is secreted in plasma where it is a component of very low density lipoprotein. This protein activates the enzyme lipoprotein lipase, which hydrolyzes triglycerides and thus provides free fatty acids for cells. Mutations in this gene cause hyperlipoproteinemia type IB, characterized by hypertriglyceridemia, xanthomas, and increased risk of pancreatitis and early atherosclerosis. This gene is present in a cluster with other related apolipoprotein genes on chromosome 19. Naturally occurring read-through transcription exists between this gene and the neighboring upstream apolipoprotein C-IV (APOC4) gene. [provided by RefSeq, Mar 2011]
Uniprot Description
APOC2: Component of the very low density lipoprotein (VLDL) fraction in plasma, and is an activator of several triacylglycerol lipases. The association of APOC2 with plasma chylomicrons, VLDL, and HDL is reversible, a function of the secretion and catabolism of triglyceride-rich lipoproteins, and changes rapidly. Defects in APOC2 are the cause of hyperlipoproteinemia type 1B (HLPP1B). It is an autosomal recessive trait characterized by hypertriglyceridemia, xanthomas, and increased risk of pancreatitis and early atherosclerosis. Belongs to the apolipoprotein C2 family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 19q13.2
Cellular Component: extracellular space; chylomicron; early endosome; extracellular region
Molecular Function: phospholipase binding; protein homodimerization activity; lipase inhibitor activity; lipid binding; phospholipase activator activity
Biological Process: phototransduction, visible light; positive regulation of fatty acid biosynthetic process; negative regulation of cholesterol transport; lipoprotein metabolic process; cholesterol efflux; cholesterol homeostasis; reverse cholesterol transport; phospholipid efflux; negative regulation of catalytic activity; positive regulation of lipoprotein lipase activity; negative regulation of receptor-mediated endocytosis; retinoid metabolic process; negative regulation of lipid metabolic process; lipid catabolic process
Disease: Apolipoprotein C-ii Deficiency
Research Articles on APOC2
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Product Notes
The Monkey APOC2 apoc2 (Catalog #AAA735398) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA735398 ELISA Kit recognizes Monkey APOC2. It is sometimes possible for the material contained within the vial of "Apolipoprotein C2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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