Typical Testing Data/Standard Curve (for reference only)
Mouse anterior pharynx defective 1 homolog B (C. elegans) ELISA Kit | APH1B elisa kit
Mouse gamma-Secretase ELISA Kit
Gene Names
Aph1b; Aph1c; 2310057K14Rik
Reactivity
Mouse
Synonyms
anterior pharynx defective 1 homolog B (C. elegans); Mouse gamma-Secretase ELISA Kit; Mouse gamma-Secretase ELISA kit; APH-1B; DKFZp564D0372; FLJ33115; PRO1328; PSFL; TAAV688; anterior pharynx defective 1B-like; presenilin stabilization factor-like; APH1B elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse gamma -Secretase. No significant cross-reactivity or interference between mouse gamma -Secretase and analogues was observed.
Sequence Length
257
Samples
Serum, plasma and tissue homogenates
Assay Type
Sandwich
Detection Range
7.8 pg/ml -500 pg/ml.
Sensitivity
The minimum detectable dose of mouse gamma -Secretase is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for APH1B elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for gamma-Secretase has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any gamma-Secretase present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for gamma-Secretase is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of gamma-Secretase bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,703 Da
NCBI Official Full Name
gamma-secretase subunit APH-1B
NCBI Official Synonym Full Names
anterior pharynx defective 1b homolog (C. elegans)
NCBI Official Symbol
Aph1b
NCBI Official Synonym Symbols
Aph1c; 2310057K14Rik
NCBI Protein Information
gamma-secretase subunit APH-1B; APH-1b; aph-1beta; APH1B homolog; anterior pharynx defective 1c homolog
UniProt Protein Name
Gamma-secretase subunit APH-1B
Protein Family
UniProt Gene Name
Aph1b
UniProt Synonym Gene Names
APH-1b
UniProt Entry Name
APH1B_MOUSE
Uniprot Description
Function: Probable subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex. Probably present in a minority of gamma-secretase complexes compared to APH1A
Research Articles on APH1B
Similar Products
Product Notes
The Mouse APH1B aph1b (Catalog #AAA700934) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA700934 ELISA Kit recognizes Mouse APH1B. It is sometimes possible for the material contained within the vial of "anterior pharynx defective 1 homolog B (C. elegans), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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