Human galactosidase, beta 1 ELISA Kit | GLB1 elisa kit

Human beta-galactosidase, betaGAL ELISA Kit

Cat. Num. AAA705153

• Gene Names: GLB1; EBP; ELNR1; MPS4B
• Reactivity: Human
• Synonyms: galactosidase; beta 1; Human beta-galactosidase; betaGAL ELISA Kit; EBP; ELNR1; elastin receptor 1; 67kDa; GLB1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human betaGAL. No significant cross-reactivity or interference between human betaGAL and analogues was observed.
• Sequence Length: 677

• Samples: Serum, plasma, urine, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 7.8 ulU/ml-500 ulU/ml.
• Sensitivity: The minimum detectable dose of human betaGAL is typically less than 1.95 ulU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GLB1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for betaGAL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any betaGAL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for betaGAL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of betaGAL bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 119372308
• NCBI GeneID: 2720
• NCBI Accession #: NP_000395.2
• NCBI GenBank Nucleotide #: NM_000404.2
• UniProt Accession #: P16278; P16279; B2R7H8; B7Z6B0
• Molecular Weight: 76,075 Da
• NCBI Official Full Name: beta-galactosidase isoform a preproprotein
• NCBI Official Synonym Full Names: galactosidase, beta 1
• NCBI Official Symbol: GLB1
• NCBI Official Synonym Symbols: EBP; ELNR1; MPS4B
• NCBI Protein Information: beta-galactosidase; lactase; acid beta-galactosidase; elastin receptor 1, 67kDa
• UniProt Protein Name: Beta-galactosidase
• Protein Family: Globulin
• UniProt Gene Name: GLB1
• UniProt Synonym Gene Names: ELNR1; Lactase
• UniProt Entry Name: BGAL_HUMAN

NCBI Description

This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the terminal beta-galactose from ganglioside substrates and other glycoconjugates. Defects in this gene are the cause of GM1-gangliosidosis and Morquio B syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

Function: Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.1 PublicationManual assertion based on experiment in:Ref.9

Catalytic activity: Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.