Human V-set domain containing T cell activation inhibitor 1 ELISA Kit | VTCN1 elisa kit

Human B7-H4 ELISA Kit

Cat. Num. AAA705429

• Gene Names: VTCN1; B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291
• Reactivity: Human
• Synonyms: V-set domain containing T cell activation inhibitor 1; Human B7-H4 ELISA Kit; B7-H4; B7H4; B7S1; B7X; B7h.5; FLJ22418; PRO1291; RP11-229A19.4; VCTN1; T cell costimulatory molecule B7x; immune costimulatory protein B7-H4; VTCN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human B7-H4. No significant cross-reactivity or interference between human B7-H4 and analogues was observed.
• Sequence Length: 187

• Samples: Serum, plasma, cell culture supernates and tissue homogenates
• Assay Type: Sandwich
• Detection Range: 7.8 ng/ml -500 ng/ml.
• Sensitivity: The minimum detectable dose of human B7-H4 is typically less than 1.95 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VTCN1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for B7-H4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any B7-H4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for B7-H4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of B7-H4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 359718944
• NCBI GeneID: 79679
• NCBI Accession #: NP_001240778.1
• NCBI GenBank Nucleotide #: NM_001253849.1
• UniProt Accession #: Q7Z7D3; Q0GN76; Q45VN0; Q5WPZ3; Q6P097; Q9H6B2
• Molecular Weight: 30,878 Da
• NCBI Official Full Name: V-set domain-containing T-cell activation inhibitor 1 isoform 2
• NCBI Official Synonym Full Names: V-set domain containing T cell activation inhibitor 1
• NCBI Official Symbol: VTCN1
• NCBI Official Synonym Symbols: B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291
• NCBI Protein Information: V-set domain-containing T-cell activation inhibitor 1; B7 family member, H4; B7 superfamily member 1; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; immune costimulatory protein B7-H4
• UniProt Protein Name: V-set domain-containing T-cell activation inhibitor 1
• Protein Family: V-set domain containing T-cell activation inhibitor
• UniProt Gene Name: VTCN1
• UniProt Synonym Gene Names: B7H4; UNQ659/PRO1291
• UniProt Entry Name: VTCN1_HUMAN

NCBI Description

This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

Function: Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.By similarityManual assertion inferred from sequence similarityUniProtKB:Q7TSP5