Human legumain ELISA Kit | LGMN elisa kit

Human Legumain, LGMN ELISA Kit

Cat. Num. AAA931900

• Gene Names: LGMN; AEP; LGMN1; PRSC1
• Reactivity: Human
• Synonyms: legumain; Human Legumain; LGMN ELISA Kit; Human Legumain (LGMN) ELISA kit; AEP; LGMN1; PRSC1; asparaginyl endopeptidase; cysteine protease 1; protease; cysteine; 1 (legumain) ; LGMN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human LGMN. No significant cross-reactivity or interference between human LGMN and analogues was observed.
• Sequence Length: 433

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 7.8 pg/ml -500 pg/ml.
• Sensitivity: The minimum detectable dose of human LGMN is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LGMN elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LGMN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LGMN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGMN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGMN bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 56682964
• NCBI GeneID: 5641
• NCBI Accession #: NP_001008530.1
• NCBI GenBank Nucleotide #: NM_001008530.2
• UniProt Accession #: Q99538; O00123; Q9BTY1
• Molecular Weight: 49,411 Da
• NCBI Official Full Name: legumain preproprotein
• NCBI Official Synonym Full Names: legumain
• NCBI Official Symbol: LGMN
• NCBI Official Synonym Symbols: AEP; LGMN1; PRSC1
• NCBI Protein Information: legumain; cysteine protease 1; protease, cysteine 1; asparaginyl endopeptidase; protease, cysteine, 1 (legumain)
• UniProt Protein Name: Legumain
• Protein Family: Legumain
• UniProt Gene Name: LGMN
• UniProt Synonym Gene Names: PRSC1
• UniProt Entry Name: LGMN_HUMAN

NCBI Description

This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008]

Uniprot Description

LGMN: Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Belongs to the peptidase C13 family.

Protein type: Protease; EC 3.4.22.34

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: lysosomal lumen; lysosome; apical part of cell; late endosome

Molecular Function: peptidase activity; cysteine-type endopeptidase activity

Biological Process: steroid metabolic process; renal system process; negative regulation of multicellular organism growth; proteolysis involved in cellular protein catabolic process; receptor catabolic process; toll-like receptor signaling pathway; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class II; negative regulation of neuron apoptosis; response to acidity; proteolysis; vitamin D metabolic process