Adenomatosis Polyposis Coli Protein (APC) Recombinant Protein | APC recombinant protein

Recombinant Adenomatosis Polyposis Coli Protein (APC)

Cat. Num. AAA2010673

• Gene Names: Apc; CC1; Min; mAPC; AI047805; AU020952; AW124434
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Adenomatosis Polyposis Coli Protein (APC); Recombinant Adenomatosis Polyposis Coli Protein (APC); APC recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SGAES KPLIYQMAPP VSKTEDVWVR IEDCPINNPR SGRSPTGNTP PVIDSVSEKG SSSIKDSKDS KDTHGKQSVG SGSPVQTVGL ETRLNSFVQV EAPEQKGTEA KPGQSNPVSI AETAETCIAE RTPFSSSSSS KHSSPSGTVA ARVTPFNYNP SPRKSSADST SARPSQIPTP VSTNTKKRDS KTDITESSGA QSPKRHSGSY LVTSV
• Sequence Length: 2845

• Applicable Applications for APC recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ser2636~Val2845 (Accession # Q61315) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 12643510
• NCBI GeneID: 11789
• UniProt Accession #: Q61315; Q62044
• Molecular Weight: 25.9kDa
• NCBI Official Full Name: Adenomatous polyposis coli protein
• NCBI Official Synonym Full Names: adenomatosis polyposis coli
• NCBI Official Symbol: Apc
• NCBI Official Synonym Symbols: CC1; Min; mAPC; AI047805; AU020952; AW124434
• NCBI Protein Information: adenomatous polyposis coli protein; multiple intestinal neoplasia
• UniProt Protein Name: Adenomatous polyposis coli protein
• Protein Family: Adenomatous polyposis coli
• UniProt Gene Name: Apc
• UniProt Synonym Gene Names: Protein APC; mAPC
• UniProt Entry Name: APC_MOUSE

Uniprot Description

APC: a tumor suppressor. Defects in APC are a cause of familial adenomatous polyposis (FAP). Promotes rapid degradation of CTNNB1 and participates in Wnt signaling. Activity is correlated with its phosphorylation state. Two splice-variant isoforms have been described.

Protein type: Tumor suppressor; Motility/polarity/chemotaxis

Cellular Component: centrosome; microtubule; dendrite; beta-catenin destruction complex; kinetochore; cell-cell adherens junction; cell soma; membrane; lamellipodium; cytoplasmic microtubule; cell projection membrane; cytoplasm; synapse; lateral plasma membrane; neuron projection; nuclear membrane; protein complex; tight junction; cell; cell cortex; cell projection; growth cone; cytoskeleton; axon; plasma membrane; nucleus; cell junction

Molecular Function: microtubule plus-end binding; protein binding; protease binding; microtubule binding; gamma-catenin binding; protein complex binding; beta-catenin binding; protein kinase regulator activity; protein kinase binding

Biological Process: skin development; negative regulation of Wnt receptor signaling pathway; positive regulation of cell adhesion; positive regulation of apoptosis; somatic stem cell maintenance; regulation of cell cycle; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; regulation of cell migration; cytoplasmic microtubule organization and biogenesis; anterior/posterior pattern formation; muscle maintenance; hair follicle development; cytokinesis after mitosis; positive regulation of epithelial cell differentiation; protein complex assembly; cell cycle arrest; kidney development; neurite development; regulation of microtubule-based process; negative regulation of odontogenesis; regulation of osteoblast differentiation; negative regulation of cyclin-dependent protein kinase activity; retina development in camera-type eye; positive regulation of cell division; regulation of epithelial cell differentiation; positive regulation of protein catabolic process; regulation of nitrogen compound metabolic process; positive regulation of cell differentiation; mitotic metaphase/anaphase transition; negative regulation of apoptosis; positive regulation of microtubule polymerization; establishment and/or maintenance of cell polarity; chromosome organization and biogenesis; negative regulation of cell proliferation; regulation of osteoclast differentiation; regulation of cell differentiation; mitotic cell cycle spindle assembly checkpoint; proximal/distal pattern formation; negative regulation of epithelial cell proliferation; cell migration; regulation of attachment of spindle microtubules to kinetochore; Wnt receptor signaling pathway; thymus development; negative regulation of microtubule depolymerization; negative regulation of MAPKKK cascade; axis specification; stem cell maintenance; pattern specification process; positive regulation of pseudopodium formation; axonogenesis; dorsal/ventral pattern formation; response to DNA damage stimulus; positive regulation of cell migration