Adenomatosis Polyposis Coli Protein (APC) Recombinant Protein | APC recombinant protein

Recombinant Adenomatosis Polyposis Coli Protein (APC)

Cat. Num. AAA2009367

• Gene Names: Apc; RATAPC
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Adenomatosis Polyposis Coli Protein (APC); Recombinant Adenomatosis Polyposis Coli Protein (APC); APC recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-AASGAES KTLIYQMAPA VSRTEDVWVR IEDCPINNPR SGRSPTGNTP PVIDSISEKG NPSIKDSKDT QGKQSVGSGS PVQTVGLENR LNSFIQVEAP EQKGTETKAG QGSPAPVAET GETCMAERTP FSSSSSSKHS SPSGTVAARV TPFNYNPSPR KSSADSTSAR PSQIPTPVGS STKKRDSKTD STESSGAQSP KRHSGSYLVT SV
• Sequence Length: 2842

• Applicable Applications for APC recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Ala2634~Val2842 (Accession # P70478) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6978509
• NCBI GeneID: 24205
• NCBI Accession #: NP_036631.1
• NCBI GenBank Nucleotide #: NM_012499.1
• UniProt Accession #: P70478
• Molecular Weight: 25.6kDa
• NCBI Official Full Name: adenomatous polyposis coli protein
• NCBI Official Synonym Full Names: adenomatous polyposis coli
• NCBI Official Symbol: Apc
• NCBI Official Synonym Symbols: RATAPC
• NCBI Protein Information: adenomatous polyposis coli protein; adenomatosis polyposis coli
• UniProt Protein Name: Adenomatous polyposis coli protein
• Protein Family: Adenomatous polyposis coli
• UniProt Gene Name: Apc
• UniProt Synonym Gene Names: Protein APC
• UniProt Entry Name: APC_RAT

NCBI Description

binds microtubules; may play a role in the regulation of cell polarity [RGD, Feb 2006]

Uniprot Description

APC: a tumor suppressor. Defects in APC are a cause of familial adenomatous polyposis (FAP). Promotes rapid degradation of CTNNB1 and participates in Wnt signaling. Activity is correlated with its phosphorylation state. Two splice-variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Tumor suppressor

Cellular Component: microtubule; centrosome; nuclear membrane; neuron projection; tight junction; protein complex; cell; dendrite; cell cortex; beta-catenin destruction complex; kinetochore; cell projection; cell-cell adherens junction; growth cone; cell soma; cytoplasmic microtubule; lamellipodium; axon; cell projection membrane; cytoplasm; plasma membrane; synapse; nucleus; lateral plasma membrane

Molecular Function: microtubule plus-end binding; protein binding; protease binding; cadherin binding; gamma-catenin binding; microtubule binding; protein complex binding; beta-catenin binding; protein kinase regulator activity; protein kinase binding

Biological Process: skin development; negative regulation of Wnt receptor signaling pathway; positive regulation of cell adhesion; regulation of cell cycle; somatic stem cell maintenance; positive regulation of apoptosis; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; regulation of cell migration; cytoplasmic microtubule organization and biogenesis; anterior/posterior pattern formation; muscle maintenance; hair follicle development; cytokinesis after mitosis; positive regulation of epithelial cell differentiation; protein complex assembly; cell cycle arrest; kidney development; neurite development; response to drug; regulation of microtubule-based process; negative regulation of odontogenesis; regulation of osteoblast differentiation; negative regulation of cyclin-dependent protein kinase activity; retina development in camera-type eye; positive regulation of cell division; regulation of epithelial cell differentiation; positive regulation of protein catabolic process; regulation of nitrogen compound metabolic process; positive regulation of cell differentiation; mitotic metaphase/anaphase transition; negative regulation of apoptosis; positive regulation of microtubule polymerization; establishment and/or maintenance of cell polarity; chromosome organization and biogenesis; negative regulation of cell proliferation; regulation of osteoclast differentiation; regulation of cell differentiation; mitotic cell cycle spindle assembly checkpoint; pancreas development; proximal/distal pattern formation; negative regulation of epithelial cell proliferation; regulation of attachment of spindle microtubules to kinetochore; cell migration; Wnt receptor signaling pathway; thymus development; negative regulation of microtubule depolymerization; negative regulation of MAPKKK cascade; axis specification; stem cell maintenance; pattern specification process; positive regulation of pseudopodium formation; dorsal/ventral pattern formation; axonogenesis; response to DNA damage stimulus; positive regulation of cell migration