TTK Protein Kinase (TTK) Recombinant Protein | TTK recombinant protein

Recombinant TTK Protein Kinase (TTK)

Cat. Num. AAA2012041

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: TTK Protein Kinase (TTK); Recombinant TTK Protein Kinase (TTK); TTK recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-E ITDRYIYMVM ECGNIDLNSW LKKKKSINPW ERKSYWKNML EAVHTIHQHG IVHSDLKPAN FLIVDGMLKL IDFGIANQMQ PDTTSIVKDS QVGTVNYMAP EAIRDMSSSR ENGKIKTKIS PRSDVWSLGC ILYYMTYGKT PFQHIINQVS KLHAIIDPSH EIEFPEISEK DLRDVLKCCL VRNPKERISI PELLAHPYVQ IQPHPGSQMA KETTDEM
• Sequence Length: 835

• Applicable Applications for TTK recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Glu570~Met787 (Accession # D4A4S7) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 7.3
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 157823481
• NCBI GeneID: 315852
• NCBI Accession #: NP_001101642.1
• NCBI GenBank Nucleotide #: NM_001108172.1
• UniProt Accession #: D4A4S7
• Molecular Weight: 28.9kDa
• NCBI Official Full Name: dual specificity protein kinase TTK
• NCBI Official Synonym Full Names: Ttk protein kinase
• NCBI Official Symbol: Ttk
• NCBI Protein Information: dual specificity protein kinase TTK
• UniProt Protein Name: Protein Ttk
• Protein Family: Protein tramtrack
• UniProt Gene Name: Ttk
• UniProt Synonym Gene Names: Ttk_predicted
• UniProt Entry Name: D4A4S7_RAT

Uniprot Description

TTK: a dual-specificity protein kinase orthologous to the yeast monopolar spindle (Mps1) kinase that is required for spindle pole duplication and the spindle checkpoint but not for centrosome duplication. Localized to centrosomes, kinetochores and nuclear pores. Its enzymatic activity is required for the recruitment of Mad1, Mad2, Bub1 and Bub3 to the kinetochore. Mps1 mRNA levels change little throughout the cell cycle, but its protein expression varies during the cell cycle. Overexpression arrests cells in metaphase by activating the checkpoint.

Protein type: Protein kinase, dual-specificity (non-receptor); EC 2.7.12.1; Cancer Testis Antigen (CTA); Protein kinase, Other; Kinase, protein; Other group; TTK family

Cellular Component: membrane

Molecular Function: protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; ATP binding

Biological Process: mitotic cell cycle checkpoint; protein amino acid phosphorylation; chromosome separation