Lysyl Oxidase Like Protein 1 (LOXL1) Recombinant Protein | LOXL1 recombinant protein

Recombinant Lysyl Oxidase Like Protein 1 (LOXL1)

Cat. Num. AAA2012267

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Lysyl Oxidase Like Protein 1 (LOXL1); Recombinant Lysyl Oxidase Like Protein 1 (LOXL1); LOXL1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-VYRPNQNG RGLPDLVPDP NYVQASTYVQ RAHLYSLRCA AEEKCLASTA YAPEATDYDL RVLLRFPQRV KNQGTADFLP NRPRHTWEWH SCHQHYHSMD EFSHYDLLDA STGKKVAEGH KASFCLEDST CDFGNLKRYA CTSHTQGLSP GCYDTYNADI DCQWIDITDV QPGNYILKVH VNPKYIVLES DFTNNVVRCN IHYTGRFVST TNC
• Sequence Length: 608

• Applicable Applications for LOXL1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Val393~Cys603 (Accession # Q5FWS5) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 58865820
• NCBI GeneID: 315714
• NCBI Accession #: NP_001012125.1
• NCBI GenBank Nucleotide #: NM_001012125.1
• UniProt Accession #: Q5FWS5; E9PTE3
• Molecular Weight: 28.0kDa
• NCBI Official Full Name: lysyl oxidase homolog 1
• NCBI Official Synonym Full Names: lysyl oxidase-like 1
• NCBI Official Symbol: Loxl1
• NCBI Protein Information: lysyl oxidase homolog 1
• UniProt Protein Name: Lysyl oxidase-like 1
• Protein Family: Lysyl oxidase
• UniProt Gene Name: Loxl1
• UniProt Entry Name: Q5FWS5_RAT

Uniprot Description

LOXL1: Active on elastin and collagen substrates. Genetic variations in LOXL1 are a cause of susceptibility to exfoliation syndrome (XFS); also called exfoliation glaucoma (XFG). XFS is a disorder characterized by accumulation of abnormal fibrillar deposits in the anterior segment of the eye. In addition to being a cause of glaucoma and glaucomatous optic neuropathy, exfoliation syndrome has also been associated with lens zonule weakness, cataract formation, and systemic vascular complications due to deposition of exfoliation material in extraocular tissues. Susceptibility to exfoliation syndrome is conferred by a risk haplotype that includes two LOXL1 coding non-synonymous SNPs (Arg141Leu and Gly153Asp) and one intronic SNP. Arg141Leu and Gly153Asp are sufficient to confer disease susceptibility in some populations. Belongs to the lysyl oxidase family.

Protein type: Extracellular matrix; Secreted, signal peptide; Oxidoreductase; Secreted; EC 1.4.3.-

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; cytoplasm; acrosome; basement membrane

Molecular Function: copper ion binding; oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor

Biological Process: response to lipopolysaccharide