T Cell Signaling Antibody Kit

T Cell Signaling Antibody Sampler Kit

Cat. Num. AAA2550095

• Applications: Western Blot
• Synonyms: T Cell Signaling; T Cell Signaling Antibody Sampler Kit; T Cell Signaling antibody kit

• Applicable Applications for T Cell Signaling antibody kit: Western Blot (WB)

Western Blot (WB)

(Western Blot analysis of 293 cells with Phospho-PLC gamma1 (Tyr783) Polyclonal Antibody)

Western Blot (WB)

(Western Blot analysis of 293 cells with Phospho-c-Src (Tyr419) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of K562 cells with Phospho-SLP-76 (Tyr128) Polyclonal Antibody)

Western Blot (WB)

(Western Blot analysis of 3T3 cells with Phospho-Syk (Tyr348) Polyclonal Antibody)

Related Product Information for T Cell Signaling antibody kit

Background: When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery. This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR alpha and beta chains. CD3 is composed of four polypeptides: zeta, gamma, epsilon and delta. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM). The Src family kinases Lck and Fyn are recruited to the TCR complex upon stimulation and activate the downstream tyrosine kinases to initiate signaling. Phosphorylation of Lck at Tyr394 leads to an increase in Lck activity while phosphorylation of Tyr505 in the Lck carboxy- terminal tail down-regulates Lck catalytic activity. Zap-70 and Syk are rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by Src family tyrosine kinases. Activation loop phosphorylation of Zap-70 at Tyr493 and Syk at Tyr526 leads to complete activation of both kinases. Subsequent phosphorylation of other tyrosine residues within the kinase interdomain B region, including Zap-70 at Tyr315 and Zap-70 at Tyr 319, create docking sites for downstream signaling molecules. Zap-70 and Syk phosphorylate the transmembrane adaptor protein LAT at multiple, conserved tyrosine residues within SH2 binding motifs, exposing these motifs as docking sites for downstream signaling targets. The phosphorylation of LAT at Tyr171 and Tyr191 enables the binding of Grb2, Gads/SLP-76, PLCgamma1, and PI3 kinase. The adapter protein SLP-76 is phosphorylated at Tyr113 and Tyr128, allowing for binding of the Grb2-like adapter Gads. Phosphorylation of SLP-76 at Ser376 by hematopoietic progenitor kinase 1 (HPK1) induces interaction with 14-3-3epsilon and down-regulates TCR signaling. Phosphoinositide-specific phospholipase PLCgamma1 enzyme activity is also stimulated by Zap-70 and Syk phosphorylation on Tyr783, Tyr711, and Tyr1253, resulting in robust PI-4,5-P2 hydrolysis.