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Western Blot (WB) (Western Blot analysis of HuvEc cells with Phospho-Flk-1 (Tyr1175) Polyclonal Antibody at dilution of 1:1000)

Angiogenesis Antibody Kit

Angiogenesis Antibody Sampler Kit

Applications
Western Blot
Synonyms
Angiogenesis; Angiogenesis Antibody Sampler Kit; Angiogenesis antibody kit
Ordering
For Research Use Only!
Applicable Applications for Angiogenesis antibody kit
Western Blot (WB)

Western Blot (WB)

(Western Blot analysis of HuvEc cells with Phospho-Flk-1 (Tyr1175) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB) (Western Blot analysis of HuvEc cells with Phospho-Flk-1 (Tyr1175) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of 3T3 cells treated with PDGF with Phospho-Akt (Ser473) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB) (Western Blot analysis of 3T3 cells treated with PDGF with Phospho-Akt (Ser473) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of 293 cells with Phospho-c-Src (Tyr419) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB) (Western Blot analysis of 293 cells with Phospho-c-Src (Tyr419) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of COLO205 cells with Phospho-c-Src (Ser75) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB) (Western Blot analysis of COLO205 cells with Phospho-c-Src (Ser75) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of 293T cells with Phospho-FAK (Tyr397) Polyclonal Antibody)

Western Blot (WB) (Western Blot analysis of 293T cells with Phospho-FAK (Tyr397) Polyclonal Antibody)

Western Blot (WB)

(Western Blot analysis of various cells with Phospho-p38 (Thr180/Tyr182) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB) (Western Blot analysis of various cells with Phospho-p38 (Thr180/Tyr182) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of 293 cells with Phospho-PLC gamma1 (Tyr783) Polyclonal Antibody)

Western Blot (WB) (Western Blot analysis of 293 cells with Phospho-PLC gamma1 (Tyr783) Polyclonal Antibody)

Western Blot (WB)

(Western Blot analysis of Hela, 293 cells with Phospho-ERK 1/2 (Thr202/Tyr204) Polyclonal Antibody)

Western Blot (WB) (Western Blot analysis of Hela, 293 cells with Phospho-ERK 1/2 (Thr202/Tyr204) Polyclonal Antibody)
Related Product Information for Angiogenesis antibody kit
Background: Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated. Signaling from VEGFR2 is necessary for angiogenesis in vivo. Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2. Phosphorylation of VEGFR2 at Tyr1212 provides a docking site for GRB2 binding and phosphorylation at Tyr1175 binds the p85 subunit of PI3 kinase and PLCgamma. Activation of VEGFR2 during angiogenesis leads to signaling through multiple downstream kinase pathways including Akt, Src, FAK, p38, and Erk1/2.

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Product Notes

The Angiogenesis (Catalog #AAA2549980) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Angiogenesis can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Angiogenesis for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Angiogenesis, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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